The inhibitory effect of Sambiloto (Andrographis paniculata Nees) leaf extract on growth of Candida albicans
1 Department of Periodontology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
2 Dental Medicine Education Study Program, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
Research Article
World Journal of Advanced Research and Reviews, 2023, 20(03), 752–758
Article DOI: 10.30574/wjarr.2023.20.3.2521
Publication history:
Received on 31 October 2023; revised on 09 December 2023; accepted on 11 December 2023
Abstract:
Background: Linear gingival erythema (LGE) is a periodontal disease caused by fungal infection of the genus Candida. Based on the results of Nugraha’s research, the most common Candida species in LGE was Candida albicans which reached 71.43%. Several studies have shown that sambiloto (Andrographis paniculata Nees) has antifungal activity because sambiloto contains active ingredients such as andrographolides, flavonoids, alkaloids, saponins, and tannins.
Objective: This study aims to determine the inhibitory effect of sambiloto (Andrographis paniculata Nees) leaf extract on growth of Candida albicans.
Methods: This research is an in-vitro laboratory experimental study. Sambiloto (Andrographis paniculata Nees) leaf extract was produced by the maceration method using 70% ethanol. The inhibition testing on Candida albicans cultures was carried out by spectrophotometric methods and colony counts.
Result: At concentration of 100%, 50%, 25%, and 12.5% of sambiloto leaf extract, no fungal colonies were found. While at concentration of 6.25% can inhibit fungal by 91.67%. Statistical tests on each concentration group showed a significance value of 0,001 (p<0.05).
Conclusion: Sambiloto (Andrographis paniculata Nees) leaf extract has an inhibitory effect on growth of Candida albicans with MIC values at concentration of 6.125% and MBC of 12.5%.
Keywords:
Sambiloto (Andrographis paniculata Nees); Candida albicans; Inhibitory effect; Spectrophotometry; Colony counts.
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