Effect of Alpha Lipoic Acid (ALA) Supplementation in Goat Oocyte In Vitro Maturation Media on Superoxide Dismutase (SOD) and 8-Hydroxy-2'-Deoxyguanosine (8-OHdG) Levels
1 Magister of Reproductive Biology, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, East Java, Indonesia.
2 Department of Veterinary Science, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, East Java, Indonesia.
Research Article
World Journal of Advanced Research and Reviews, 2023, 20(02), 397–403
Article DOI: 10.30574/wjarr.2023.20.2.2292
Publication history:
Received on 23 September 2023; revised on 06 November 2023; accepted on 08 November 2023\
Abstract:
Introduction: This study explains about the effect of Alpha Lipoic Acid (ALA) supplementation in goat oocyte In Vitro maturation media on Superoxide Dismutase (SOD) levels and 8-Hydroxy-2'-Deoxyguanosine (8-OHdG) levels with the aim of determining the status of balanced intracellular redox status and good oocyte quality.
Objective: This laboratory experimental study contained three treatment groups: no ALA (P0), 25 μmol/L ALA supplementation (P1), and 50 μmol/L ALA supplementation (P2). SOD and 8-OHdG levels were measured using the ELISA method. SOD levels in group P0 were 1.41±0.40 pg/mL, group P1 were 2.04±0.50 pg/mL, and group P2 were 3.65±1.20 pg/mL. Data on 8-OHdG levels in the P0 group was 51.17±27.58 pg/mL, the P1 group was 11.21±5.98 pg/mL, and the P2 group was 12.66±6.01 pg/mL.
Results: The P1 group could not significantly increase the SOD level of the P0 group (p>0.05), while the P2 group could significantly increase the SOD level of the P0 group (p<0.05). Data on 8-OHdG levels in the P1 and P2 groups decreased significantly when compared to the P0 group (p<0.05).
Conclusion: ALA supplementation at a dose of 50 µmol/L in goat oocyte In Vitro maturation media can increase SOD levels and reduce 8-OHdG levels.
Keywords:
Oocyte; Alpha Lipoic Acid; In Vitro Maturation; SOD; 8-OHdG
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