Effect of different face washes on the bacterial pathogens of skin

Mubeen Mahabub Basha 1, Bharathi Balasubramanian 2, * and Deepa C. Philip 3

1 Medical Lab technology, MMM College of Health Sciences. Chennai, Tamil Nadu, India.
2 Department of Medical Lab technology, MMM College of Health Sciences, Chennai, Tamil Nadu, India.
3 Principal, MMM College of Health Sciences. Chennai, Tamil Nadu, India.
 
Research Article
World Journal of Advanced Research and Reviews, 10.30574/wjarr.2023.20.2.2139
Article DOI: 10.30574/wjarr.2023.20.2.2139
 
Publication history: 
Received on 11 September 2023; revised on 25 October 2023; accepted on 28 October 2023
 
Abstract: 
Pathogens like Propionibacterium acnes, Staphylococcus aureus, E. coli, and Candida species are kept at bay by the skin's natural flora. There are face washes and cleansers on the market now that eliminate specific skin germs and keep healthy skin infection-free. Each volunteer's face was used to take a sample, the sample was taken by gently scraping every surface of the participant's face, including the popped-up Acne, using a sterile swab dipped in normal saline. The gathered samples were cultured on Nutrient Agar, and the organisms that had developed there were recognised by Gram staining and the appropriate biochemical identification and placedin different levels. The organism was then put to the test against several isolated organisms using a sensitivity test (Kirby-bauer Method) using diluted versions of 5 different face washes. Staphylococcus aureus, Micrococcus spp., Bacillus spp., E. coli, and Klebsiella spp., were among the predominant strains among the 40 different strains that were recovered from the 40 different volunteers .The antimicrobial effects of five commercially available face washes Vicco, Cetaphil, Himalayas, Neem and Tulsi and Ponds were tested on the isolated organisms at dilutions of 1:10. When compared to other face washes, HIMALAYA has the strongest antibacterial activity and the second- highest maximum zone of inhibition against the following microorganisms: Staphylococcus aureus, Micrococci spp., and Klebsiella spp., According to the experiment, face washes PONDS and CETAPHIL have the least antibacterial activity, no zone of inhibition, and are resistant to all five detected organisms.
 
Keywords: 
Face washes; Acne; Kirby-bauer Method; Zone of inhibition; Resistance; Grams staining; Biochemical reaction; Micrococci; Klebsiella
 
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