Cytotoxicity of chitosan gelatin limestone-based carbonate hydroxyapatite composite scaffold after crosslinking

Background: The development of biomaterial scaffolds is used as one of the solutions for dealing with bone damage. The combination of materials in scaffolds before being applied to clinical practice therefore required several stages for testing the biocompatibility material. One of the material biocompatibility tests involves a cytotoxicity test on osteoblast 7F2 cells. Purpose: To demonstrates the percentage of viable cells for Chitosan-Gelatin:Carbonate HydroxyApatite (C G:CHA) scaffold with a ratio of 20:80, 30:70, and 40:60 (w:w) after crosslinking with 0.25% glutaraldehyde on 7F2 osteoblasts. Methods: MTT assay is a cytotoxicity test used in this research. The research data are analyzed by comparative tests using Shapiro-Wilk, Levene, and One-Way Anova test, post hoc Games-Howell. Results: Statistical tests from the percentage of live cells shows that between the control cell group and the C-G:CHA 20:80 crosslink group, the cell group and the C-G:CHA 20:80 group without crosslink, the C G:CHA group 20:80 crosslink and C-G:CHA group 30:70 without crosslink, C-G:CHA group 20:80 without crosslink and C-G:CHA group 30:70 without crosslink, there are significant differences. Conclusion: Scaffold C-G:CHA with a ratio of 20:80, 30:70, and 40:60 (w:w) after crosslinked with 0.25% glutaraldehyde was found not toxicity to osteoblast cells 7F2 because indirectly cytotoxicity test resulted a percentage of live cells of >50%.