Phytochemical screening: Determination of total polyphenol and flavonoid contents, antioxidant and antimicrobial activity of leaves and seeds of Abelmoschus esculentus L.
Department of Chemistry, Faculty of Sciences and Technology, University Cheikh Anta Diop, Dakar-Sénégal.
Research Article
World Journal of Advanced Research and Reviews, 2023, 19(01), 394–402
Publication history:
Received on 25 May 2023; revised on 02 July 2023; accepted on 04 July 2023
Abstract:
Reputed to be a plant rich in antioxidants, Abelmoschus esculentus or okra is a very effective vegetable against diabetes. In this work, we made a qualitative study of the bioactive compounds contained in the leaves and seeds of okra, through a phytochemical screening. Subsequently, the antioxidant and antimicrobial activities of these extracts were studied. The Folin-Ciocalteu reagent was used to evaluate the content of the phenolic compound. The DPPH● radical made it possible to measure the antioxidant power of the extracts. The phytochemical analysis made it possible to high the presence of substances with great therapeutic values (polyphenols, flavonoids, tannins, alkaloids, etc.). The antibacterial activity was measured on bacterial strains. The antioxidant capacities reveal that the extracts of the leaves and seeds of okra possess an interesting anti-free radical activity with IC50 values of 06 ± 0.21 mg / mL and 8.85 ± 0.28 mg / mL respectively for the leaves and the seeds. . Biological analysis shows that the E. faecalis strain is resistant to different extracts. E. coli, S. aureus, and P. aeruginosa strains show some sensitivity to ethanolic extracts. However, E. colis and S. aureus strains show the best antimicrobial activities with a MIC of 3.75 mg/mL for crude ethanol extract and ethanol fraction of leaves and seeds.
Keywords:
Abelmoschus esculentus L; Phytochemical screening; Polyphenols; Flavonoids; Antioxidant; Antimicrobial.
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Copyright © 2023 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0