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eISSN: 2581-9615 || CODEN: WJARAI || Impact Factor 8.2 ||  CrossRef DOI

Research and review articles are invited for publication in March 2026 (Volume 29, Issue 3) Submit manuscript

Secretion of osteocalcin in chitosan–hydroxyapatite scaffold with seeding of cryopreserved human adipose derived mesenchymal stem cells

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  • Secretion of osteocalcin in chitosan–hydroxyapatite scaffold with seeding of cryopreserved human adipose derived mesenchymal stem cells

Michael Josef Kridanto Kamadjaja 1, Chandrasasi Berlian Pratiwi 2, *, Elmira Zuhuro Putri Afandi 2 and Shima Ayu Nourma Kholifah 2

1 Department of Prosthodontics, Faculty of Dental Medicine, Airlangga University, Surabaya, Indonesia.
2 Faculty of Dental Medicine, Airlangga University, Surabaya, Indonesia.
 
Research Article
World Journal of Advanced Research and Reviews, 2024, 23(02), 297-304
Article DOI: 10.30574/wjarr.2024.23.2.2314
DOI url: https://doi.org/10.30574/wjarr.2024.23.2.2314
 
Received on 23 June 2024; revised on 02 August 2024; accepted on 04 August 2024
 
Background: Tissue engineering has developed in recent years, including the utilization of bone graft to regenerate bone. The incorporation of chitosan and hydroxyapatite as scaffolds improves osteoconductive ability. Human adipose derived mesenchymal stem cells (hADMSCs) potentially differentiate into osteoblasts and can accelerate the osteogenesis process. 
Purpose: To determine osteocalcin secretion on chitosan-hydroxyapatite (CS-HA) scaffold with seeding of cryopreserved hADMSCs in vitro
Method: Thawing was conducted on frozen (hADMSCs) and characterized using immunocytochemistry. HADMSCs were seeded on CS-HA scaffolds. Examination of osteocalcin secretion levels was carried out by collecting cell culture supernatants of hADMSCs on 7th, 14th and 21st day of the observation group by using osteocalcin ELISA kit. 
Result: Scanning observation (SEM) proved that the chitosan-hydroxyapatite scaffold has a suitable pore structure, because hADMSCs can attach and differentiate when attached to the porosity of the scaffold. There were significant differences in osteocalcin secretion on days 7, 14 and 21 according to the ELISA results. 
Conclusion: The presence of bone regeneration process in chitosan-hydroxyapatite scaffold. This was characterized by significant osteocalcin secretion on 7th, 14th and 21st day. So it can be concluded that the scaffold is compatible.
 
Bone Tissue Engineering; Chitosan-Hydroxyapatite Scaffold; Osteocalcin; Human Adipose Mesenchymal Derived Stem Cells
 
https://wjarr.com/sites/default/files/fulltext_pdf/WJARR-2024-2314.pdf

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Michael Josef Kridanto Kamadjaja, Chandrasasi Berlian Pratiwi, Elmira Zuhuro Putri Afandi and Shima Ayu Nourma Kholifah. Secretion of osteocalcin in chitosan–hydroxyapatite scaffold with seeding of cryopreserved human adipose derived mesenchymal stem cells. World Journal of Advanced Research and Reviews, 2024, 23(2), 297-304. Article DOI: https://doi.org/10.30574/wjarr.2024.23.2.2314

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