1 Institute of Microbiology, Friedrich Schiller University Jena, Fuerstengraben 1, 07743 Jena, Germany.
2 Department of Genetic Engineering and Biotechnology, University of Rajshahi. Rajshahi-6205, Bangladesh.
3 Department of Biotechnology and Genetic Engineering, Bangabandhu Sheikh Mujibur Rahman Science and Technology University, Gopalganj 8100, Dhaka, Bangladesh.
4 Department of Zoology, University of Rajshahi, Rajshahi 6205, Bangladesh.
5 Department of Microbiology, University of Rajshahi, Rajshahi 6205, Bangladesh.
Research Article
World Journal of Advanced Research and Reviews, 2024, 23(01), 2480-2491
Article DOI: 10.30574/wjarr.2024.23.1.2139
Received on 05 June 2024; revised on 22 July 2024; accepted on 25 July 2024
Objective: The present study was conducted for isolation and detection of the phyto-pathogen responsible for bacterial leaf spot disease of cucurbits as well as evaluation of its biological control techniques.
Methods: The pathogen of the disease was isolated from infected leaf of bitter gourd and cultured on Luria-Bertani (LB) growth medium. The morphological tests and biochemical tests revealed the isolated bacteria as gram negative. Furthermore, advanced molecular technique was applied to facilitate proper detection of the isolated bacteria. The PCR of the bacterial genomic DNA using specific primers generated clear band of approximately 1450 bp in gel electrophoresis.
Results: In gram staining test, the bacterial strain was found to be rod shaped and pinkish in color and Gram-negative. All the biochemical test showed positive without mannitol salt agar. The nucleotide sequencing of 16S rDNA gene of the bacterial isolate showed 86.02% similarities with the original sequence of Xanthomonas cucurbitae. In antibiotic sensitivity assay, the antibiotic cefixime showed highest 28.0±0.7 mm diameter of inhibition zone against the tested bacteria. The highest antibacterial activity was 8.0±0.5 mm zone of inhibition by of Allium sativum.
Conclusion: The present findings may be benevolent for the detection, characterization and development of biological control techniques to prevent the leaf spot disease of cucurbits crops in Bangladesh. It may help to find out the specific signaling pathway techniques for plant microbes relationship for disease formation in future.
Cucurbits; Bacterial leaf spot; Characterization; Biological control
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M. E. Khan Chowdhury, Md. Enamul Haque, Mehrun Nesa, Biswanath Sikdar and Md. Faruk Hasan. Identification and biological control of bacterial leaf spot disease of cucurbits. World Journal of Advanced Research and Reviews, 2024, 23(1), 2480-2491. Article DOI: https://doi.org/10.30574/wjarr.2024.23.1.2139