In-vitro interaction of daminozide with some amino acids

Dağ Şeker 1, * and Dere Egemen 2

1 Sivas Cumhuriyet University, Faculty of Science, Department of Biology SİVAS.
2 Uludag University, Faculty of Arts and Sciences, Department of Biology BURSA.
 
Research Article
World Journal of Advanced Research and Reviews, 2020, 05(01), 086-094
Article DOI: 10.30574/wjarr.2020.5.1.0007
 
Publication history: 
Received on 06 January 2020; revised on 13 January 2020; accepted on 22 January 2020
 
Abstract: 
In this study, the interaction of plant growth regulatory substance daminozide with alanine, arginine, glycine, glutamic acid, histidine, isoleucine, methionine, phenylalanine, threonine, tryptophan, tyrosine and valine has been investigated by thin-layer chromatography. In the experiment 100, 200 and 400 ppm doses of daminozide has been used. At the end of experiments with or without for 24 hours, in the Retention factor (Rf ) value, according to standard amino acids values, some increasing or decreasing values have been observed. Moreover some alterations in maximum absorption peaks on the spectrums taken from thin-layer cromotgraphy samples have been observed. It has been observed that there were some changes in amino acids with different daminozide doses. The obtained results show that daminozide either will be able to bound to amino acids or form new copounds under in-vitro conditions.
 
Keywords: 
Daminozide; Amino Acids; Thin-layer cromotgraphy
 
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