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eISSN: 2581-9615 || CODEN: WJARAI || Impact Factor 8.2 ||  CrossRef DOI

Research and review articles are invited for publication in March 2026 (Volume 29, Issue 3) Submit manuscript

In vitro micropropagation and mass multiplication of Stevia rebaudiana Bertoni

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  • In vitro micropropagation and mass multiplication of Stevia rebaudiana Bertoni

Vaibhav D. Misal 1, * and Ashok M. Chavan 2

1 Plant Tissue Culture Laboratory, Department of Botany, Dr. Babasaheb Ambedkar Marathwada University, Chh. Sambhajinagar (MS) India.
2 Seed Pathology and Fungal Biotechnology Laboratory, Department of Botany, Dr. Babasaheb Ambedkar Marathwada University, Chh. Sambhajinagar (MS) India.
 
Research Article
World Journal of Advanced Research and Reviews, 2024, 23(01), 250-254
Article DOI: 10.30574/wjarr.2024.23.1.2027
DOI url: https://doi.org/10.30574/wjarr.2024.23.1.2027
 
Received on 26 May 2024; revised on 01 July 2024; accepted on 04 July 2024
 
Stevia rebaudiana Bertoni, an ancient perennial herb produces steviol glycosides including stevioside and rebaudioside-A that are valuable as low calorie sweeteners, about 300-400 times sweeter than saccharose. Conventional propagation methods are not produce adequate planting material. Micro propagation is an imperative technology that could be extensively exploited to meet the growing demands of elite planting material for commercially cultivated. The prime objective of this study was to established standard protocol for in vitro regeneration and mass multiplication in Stevia. In present investigation, different concentrations of Auxin and Cytokinin were tried for optimization of shoot initiation, shoot multiplication, root initiation and callus induction of Stevia from different explant. BAP and IBA (1.0+0.5) mg/l, IBA (2.0) mg/l shows promising results of shoot multiplication and root initiation respectively.
 
Stevia rebaudiana; In vitro; Micropropagation; MS medium; Low calorie sweetener
 
https://wjarr.com/sites/default/files/fulltext_pdf/WJARR-2024-2027.pdf

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Vaibhav D. Misal and Ashok M. Chavan. In vitro micropropagation and mass multiplication of Stevia rebaudiana Bertoni. World Journal of Advanced Research and Reviews, 2024, 23(1), 250-254. Article DOI: https://doi.org/10.30574/wjarr.2024.23.1.2027

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