Development and validation for the simultaneous estimation of olmesartan medoxomil and hydrochlorothiazide by using RP-HPLC methods
1 Department of Pharmaceutical Analysis, Vasavi institute of pharmaceutical sciences, Vasavi Nagar, Peddapalli (Village), Sidhout (Mandal), YSR Kadapa (Dist), Andhra Pradesh-516247, India.
2 Department of Pharmaceutical Analysis and Quality Assurance, Siddahartha Institute of Pharmaceutical Sciences, Jonnalagadda Village, Narasaraopeta Mandal, Guntur District, Andhra Pradesh-522601, India.
3 Department of Pharmaceutics, Vasavi institute of pharmaceutical sciences, Vasavi Nagar, Peddapalli (Village), Sidhout (Mandal), YSR Kadapa (Dist), Andhra Pradesh-516247, India.
4 Ratnam Institute of Pharmacy, Pidathapolur Village, Muthukur Mandal, SPSR Nellore District,Andhra Pradesh-524 346, India.
Research Article
World Journal of Advanced Research and Reviews, 2023, 17(02), 686–696
Article DOI: 10.30574/wjarr.2023.17.2.0247
Publication history:
Received on 30 December 2022; revised on 10 Februay 2023; accepted on 13 February 2023
Abstract:
A simple, Accurate, precise method was developed for the simultaneous estimation of the Olmesartan Medoxomil and Hydrochlorothiazide in Tablet dosage form. Chromatogram was run through Inertsil -ODS C18,250 x 4.6 mm, 5m. Mobile phase containing Methanoland Bufferin the ratio of 80:20 was pumped through column at a flow rate of 10ml/min. Optimized wavelength for Olmesartan Medoxomil and Hydrochlorothiazidewas 260nm. Retention time of Olmesartan Medoxomiland Hydrochlorothiazide were found to be 3.270min and 4.566 min. %assay was obtained as 99.91% and 99.95% for Olmesartan Medoxomiland Hydrochlorothiazide respectively. Regression equation of Olmesartan Medoxomilis y = 30712x - 31891, and y = 16499x + 8683. Of Hydrochlorothiazide.
Keywords:
Olmesartan Medoxomil; Hydrochlorothiazide; RP-HPLC; Validation; Simultaneous Estimation.
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