1 Graduate School of Dentistry (Endodontics), Osaka Dental University, 8-1 Kuzuhahanazono-cho, Hirakata-shi, Osaka 573-1121, Japan.
2 Department of Endodontics, Faculty of Dentistry, Osaka Dental University, 8-1 Kuzuhahanazono-cho, Hirakata-shi, Osaka 573-1121, Japan.
3 Department of Oral pathology, Faculty of Dentistry, Osaka Dental University, 8-1 Kuzuhahanazono-cho, Hirakata-shi, Osaka 573-1121, Japan.
Research Article
World Journal of Advanced Research and Reviews, 2020, 08(03), 392-401
Article DOI: 10.30574/wjarr.2020.8.3.0495
Received on 17 December 2020; revised on 24 December 2020; accepted on 26 December 2020
Enamel matrix derivative (EMD) is a periodontal tissue regeneration material that induced hard tissue formation. This study was performed to examine the reaction of dental pulp tissue directly capped with a synthetic peptide prepared based on EMD research, and to investigate its potential as a pulp capping material.
The experiments were performed in the maxillary left and right first molars of five 6-week-old male Sprague–Dawley rats. The exposed pulp was capped with synthetic peptide in the peptide group, but left uncapped in the control group. Two weeks later, the maxillary bone was isolated and photographed by microfocus computed tomography (CT). The opaque area and volume of the coronal pulp cavity were measured on CT images, and the area-to-volume ratio was calculated. After decalcification, tissue samples were prepared, stained with hematoxylin and eosin (H&E), and examined histopathologically.
CT images for both groups showed an opaque area in the coronal pulp cavity, and the fraction of images showing opacity was significantly higher in the peptide group (P < 0.01). A large amount of hard tissue was formed in the coronal pulp cavity in the peptide group, around which aggregation of cells with eosinophilic substances in polytopes was observed. Only slight hard tissue formation was observed in the control group. As it showed eosinophilicity on H&E staining, the synthetic peptide was suggested to be involved in hard tissue formation. Synthetic peptides can potentially be used as pulp capping materials.
Direct pulp capping; Rat molars; Enamel matrix derivative; Hard tissue formation; A Synthetic peptide
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Nozomi Matsuo, Norimasa Tsuji, Kazuya Tominaga and Hiroshi Maeda. Hard tissue inducibility of a novel synthetic peptide in rat pulp. World Journal of Advanced Research and Reviews, 2020, 8(3), 392-401. Article DOI: https://doi.org/10.30574/wjarr.2020.8.3.0495