Formulation of ointment from extract and fractions of Castanopsis costata leaves

Nasirah Maulidia Ajhar 1, Effendy De Lux Putra 2, * and Emil Salim 3

1 Postgraduate Program of Pharmaceutical Sciences, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, 20155, Indonesia.
2 Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, 20155, Indonesia.
3 Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, 20155, Indonesia.
 
Research Article
World Journal of Advanced Research and Reviews, 2023, 17(01), 230-235
Article DOI: 10.30574/wjarr.2023.17.1.0028
 
Publication history: 
Received on 27 November 2022; revised on 08 January 2023; accepted on 10 January 2023
 
Abstract: 
Background: Castanopsis costata (Blume) A. DC. or commonly known as cep-cepan is a medicinal plant widely used based on experience by Karo people in Indonesia n the Karo community for the treatment of wounds. However, no research has been done to prove this experience. Initially, the formulation of ointment extract and leaf fractions of C. costata need to be carried out.
Aim: The purpose of this study was to provide information about the chemical group content contained in C. costata leaf extract and fractions and to evaluate their ointment preparation according to the requirements of ointment preparations.
Method: The extraction of C. costata leaves using 70% ethanol using the maceration method followed by the fractionation of C. costata leaves using water, ethyl acetate, and n-hexane as solvents and phytochemical screening were carried out. Furthermore, the preparation of ointments and evaluation of extract ointment preparations and C. costata leaf fraction ointment was performed.
Result: The results indicated that the ethanol extract of C. costata contained alkaloids, flavonoids, glycosides, steroids/terpenoids, saponins and tannins, while the water fraction contained flavonoids, saponins and tannins. The ethyl acetate fraction contained flavonoids, steroids/terpenoids, saponins, and tannins and the n-hexane fraction contained alkaloids and steroids/terpenoids. The results also indicated that the extract and fraction ointment preparations and ointment fractions evaluation, including organoleptic, homogeneity, and pH, met the evaluation requirements for ointment.
Conclusion: Ethanol extract of C. costata contains alkaloids, flavonoids, glycosides, steroids/terpenoids, saponins and tannins, while the water fraction contains flavonoids, saponins and tannins. The ethyl acetate fraction contains flavonoids, steroids/terpenoids, saponins, and tannins and the n-hexane fraction contains alkaloids and steroids/terpenoids. The extract and fraction ointment preparations and ointment met the evaluation requirements for ointment.
 
Keywords: 
Castanopsis costata; Phytochemical screening; Extract; Fractions; Ointment
 
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