In vitro propagation of Byblis liniflora Salisb
1 Petrus Ky School, Campus 2, Lot E7, Street 3, Hiep Thanh 3 Residential Area, Hiep Thanh Ward, Thu Dau Mot City, Binh Duong Province.
2 Faculty of Natural Science Pedagogy, Sai Gon University, 273 An Duong Vuong Street, Ward 3, District 5, Ho Chi Minh City, Vietnam.
Research Article
World Journal of Advanced Research and Reviews, 2022, 14(03), 232–240
Article DOI: 10.30574/wjarr.2022.14.3.0495
Publication history:
Received on 26 April 2022; revised on 31 May 2022; accepted on 02 June 2022
Abstract:
This study was conducted to develop an in vitro propagation procedure for Byblis liniflora Salisb. The process of seed sterilization and in vitro sowing achieved 95% efficiency. From the material aseptic stem segments, the shoot proliferation program was determined with the rate of 33.67 shoots/explants after 4 weeks of culture on nutrient medium (1/3 strength MS basal salts plus full Morel and Wetmore’s vitamins) with addition of a mixture of cytokinin (0.5 mg/L Kin and 2 mg/L BA) and then transferred to culture for another 4 weeks on nutrient medium. The rooting program from aseptic stem segments was to culture in nutrient medium supplemented with 0.5 mg/L NAA for 8 weeks. To produce a complete plant, single shoot separated from the proliferative clusters was transferred to a culture flask containing nutrient medium and cultured for 4 weeks. The in vitro shoot developed more branches and self-rooted to form a complete plant. The suitable growing substrate in the acclimatization and outdoor stages was a mixture of peat moss and perlite (1 : 2 w/w). The climatic conditions of Ho Chi Minh City and the Southeast of Vietnam were suitable for growing Byblis liniflora trees outdoors all year round.
Keywords:
Byblis liniflora Salisb; Ho Chi Minh City; in vitro propagation; MS medium; Shoot proliferation
Full text article in PDF:
Copyright information:
Copyright © 2022 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0