Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Madurai – 625 021, India.
Research Article
World Journal of Advanced Research and Reviews, 2023, 18(03), 842-858
Received on 06 May 2023; revised on 13 June 2023; accepted on 15 June 2023
Human respiratory syncytial virus (hRSV) is one of the triple epidemic viruses that causes infections of the respiratory tract and lungs. For multiplication of the virus in human cells, its RNA polymerase is the crucial enzyme and it forms a part of a large protein (LP). The LP is a multicomponent and multifunctional protein harboring at least 3 different enzymes. The RNA polymerase belongs to RNA-dependent RNA polymerase (RdRp) (EC: 2.7.7.48) type and performs the synthesis of both mRNAs (transcription) and genomic RNA (gRNA) (replication). In addition to the RNA polymerase, the LP also harbours two more enzymes, viz. enzymes for cap addition and cap methylation of mRNAs. The polymerase domain of the LP is analyzed for its active site amino acids and its proofreading (PR) domain. Two polymerase active site regions and a DEDD-superfamily of 3’→5’ PR exonuclease active site domain are identified in the polymerase region. The signature metal-binding motifs, viz. -GDNQ- and –SDD- which are commonly found in the RdRps of all the (-) strand RNA viral pathogens are also found in the hRSV RNA polymerase. The two highly conserved polymerase catalytic core regions identified by sequence similarity are in close agreement with other DNA/RNA polymerases already reported and hence, proposed to function in the nucleotidyl transfer reactions. Presence of the two catalytic regions also suggest that the polymerase may function in a dual mode, one for transcription and the other one for replication, using the same invariant catalytic Mg2+-binding –GDNQ- and –SDD- motifs.
Human respiratory syncytial virus; RNA polymerase; Metal-binding motifs; Polymerase active sites; Proofreading exonuclease; Proofreading exonuclease active site.
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Peramachi Palanivelu. An Insight into the Active sites of the RNA Polymerase and Proofreading Exonuclease of the Human Respiratory Syncytial Virus. World Journal of Advanced Research and Reviews, 2023, 18(3), 842-858. Article DOI: https://doi.org/10.30574/wjarr.2023.18.3.1159