1 Department of Fisheries, Fish Product Technology Study Program, Sriwijaya University, Indonesia.
2 Department of Agricultural Technology, Agricultural Engineering Study Program, Sriwijaya University, Indonesia.
3 Department of Agricultural Technology, Agricultural Product Technology Study Program, Sriwijaya University, Indonesia.
4 Department of Food Technology, Faculty of Agroindustrial Technology, Padjajaran University, Indonesia.
5 Department of Nursing, Faculty of Medice, Sriwijaya University, Indonesia.
Research Article
World Journal of Advanced Research and Reviews, 2023, 18(02), 1052-1062
Article DOI: 10.30574/wjarr.2023.18.2.0888
Received on 11 April 2023; revised on 20 May 2023; accepted on 22 May 2023
Tyndallization presents potential as a cost-effective alternative sterilisation method for small-scale producers. This research aimed to assess the effectiveness of tyndallization at various temperatures in sterilising pempek, a composite food product, and its impact on product quality. The study employed the SDS-PAGE technique to investigate protein polymerisation mechanisms and FTIR to evaluate protein structure, colour, pH, and texture changes. The results indicated protein polymerisation occurred, evidenced by a decrease in Molecule High Chain (MHC) and an increase in actin, corresponding to the rising temperature and tyndallization cycles with a sinusoidal pattern. FTIR spectra demonstrated that the β-helix structure dominated secondary protein structures, consistently observed across different levels and temperature variations. This study contributes new insights into applying thermal processes in preparing composite food products. Regarding quality, tyndallization caused a shift towards a more yellow colour in the pempek and a softer texture, although there were no significant pH changes. The alterations in protein patterns resulted from the varying cycles and temperatures experienced during the tyndallization process. The findings suggest that tyndallization can be considered an effective alternative for sterilising composite products like pempek; however, its implementation still requires the appropriate combination of time, temperature, and tyndallization cycles while considering the potential impact on quality changes during the sterilisation process.
Pempek; Tyndallization; SDS-Page; FTIR
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Agus Supriadi, Daniel Saputra, Gatot Priyanto, Eka Lidiasari, Siti Nurhasanah and Sigit Purwanto. Evaluating Tyndallisation's potential for composite food products and quality considerations. World Journal of Advanced Research and Reviews, 2023, 18(2), 1052-1062. Article DOI: https://doi.org/10.30574/wjarr.2023.18.2.0888