Department of Chemistry, Faculty of Mathematic and Natural Science, Universittas Negeri Surabaya, Indonesia.
Research Article
World Journal of Advanced Research and Reviews, 2023, 17(01), 1069-1078
Article DOI: 10.30574/wjarr.2023.17.1.0100
Received on 08 December 2022; revised on 19 January 2023; accepted on 21 January 2023
The xanthine oxidase enzyme is an enzyme that catalyzes purine metabolic reactions, the oxidation of hypoxanthine to uric acid through xanthine compounds. High uric acid causes hyperuricemia. One treatment is to inhibit the xanthine oxidase enzyme. It can be conducted with a natural remedy, for example kaffir lime leaf extract. Research showed that kaffir lime leaves contain flavonoids that can inhibit the activity of the xanthine oxidase. This research aimed to examine kaffir lime leaf extract as an inhibitor of xanthine oxidase enzyme activity. Isolation of kaffir lime leaves was conducted by various maceration times of 24, 48, and 72 hours. The method of determining the inhibition value (IC50) of kaffir lime leaf extract to the xanthine oxidase enzyme was UV-Vis spectrophotometer at λ 291,5 nm. Maceration results of kaffir lime leaf extract with phytochemical tests for 48 hours showed that there were phenolic, flavonoids, steroids, alkaloids, tannins, and triterpenoids compounds. FT-IR test showed that there were C=O, C=C aromatic, O-H, and CH-aliphatic groups, which indicated the structure of the flavonoids. Maceration of kaffir lime leaf extract for 48 hours was a weak inhibitor with an IC50 value of 285,82 ppm.
Flavonoids; Kaffir lime leaf; Uric acid; Xanthine Oxidase
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Hilyatul Malikhah and Nuniek Herdyastuti. Effect of kaffir lime (Cytrus hystrix) leaf extract on xanthine oxidase inhibition. World Journal of Advanced Research and Reviews, 2023, 17(1), 1069-1078. Article DOI: https://doi.org/10.30574/wjarr.2023.17.1.0100