1 Department of Medical Microbiology, Faculty of Basic Clinical Medicine, Chukwuemeka Odumegwu Ojukwu University, Awka Campus. Anambra State, Nigeria.
2 Department of Medical Laboratory Science, Faculty of Health Sciences and Technology, Nnamdi Azikiwe University, Nnewi Campus, Anambra State, Nigeria.
3 Department of Medical Microbiology, Faculty of Health Sciences and Technology, Nnamdi Azikiwe University, Nnewi Campus, Anambra State, Nigeria.
4 Department of Medical Microbiology and Parasitology, Nnamdi Azikiwe University Teaching Hospital, Nnewi, Anambra State, Nigeria.
Research Article
World Journal of Advanced Research and Reviews, 2022, 16(03), 332-342
Article DOI: 10.30574/wjarr.2022.16.3.1290
Received on 18 October 2022; revised on 03 December 2022; accepted on 05 December 2022
Background: Parvovirus B19 infection causes a wide range of complication in pregnant women including spontaneous abortion, severe fetal anaemia, non-immune hydrops fetalis and even intrauterine fetal death, erythema infectiosum (5th) disease and aplastic crisis.
Objective: To perform molecular detection of human Parvovirus B19 among pregnant women attending antenatal clinic in Nnamdi Azikiwe Teaching Hospital Nnewi, Anambra State, Nigeria.
Materials and Methods: This is a cross-sectional study conducted among 92 consented pregnant women, between the ages of 20-40 years who are attending antenatal clinic in Nnamdi Azikiwe University Teaching Hospital Anambra State, Nigeria, from May 2020-November 2020. A total of 92 blood specimen was collected from the pregnant women.
Result: Out of the 20 IgM positive samples analyzed for Parvovirus B19 DNA, 13(65.0%) had Parvovirus B19 DNA and 7(35.0%) were negative for Parvovirus B19 DNA. Prevalence of Parvovirus B19 DNA in relation to gestational age was highest (69.2%) among pregnant women who were in their 2nd trimester and the prevalence was lowest (0.0%) among pregnant women who were in their 3rd trimester. Prevalence of Parvovirus B19 in relation to history of blood transfusion was highest (100.0%) among pregnant women who have had transfusion history and the prevalence was lowest (0.0%) among pregnant women who had no transfusion history.
Conclusion: The frequency rate of Parvovirus B19 DNA seropostivity was highest (65.0%) among the pregnant women investigated. The association of Parvovirus B19 DNA seroposivity with gestation age and history of blood transfusion was statistically significant.
Molecular detection; Parvovirus B19 DNA; Real-time PCR; Pregnant women; NAUTH Nnewi
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EUCHARIA A DILIBE, NNEKA. R AGBAKOBA, SAMUEL I OGENYI, IFEOMA G NWAFULUAKU and CHIDOZIE V UDEOGU. Molecular detection of human parvovirus B19 DNA amongst pregnant women attending antenatal clinic in Nnamdi Azikiwe University Teaching Hospital Nnewi, Anambra State, Nigeria. World Journal of Advanced Research and Reviews, 2022, 16(3), 332-342. Article DOI: https://doi.org/10.30574/wjarr.2022.16.3.1290