Bioanalytical method development and validation of UV spectrophotometric method for estimation of Bumetanide spiked in human urine
Department of Pharmaceutical Analysis, RBVRR Women’s College of Pharmacy, Barkatpura, Hyderabad, India.
Research Article
World Journal of Advanced Research and Reviews, 2021, 11(02), 241–248
Article DOI: 10.30574/wjarr.2021.11.2.0298
Publication history:
Received on 24 May 2021; revised on 26 June 2021; accepted on 28 June 2021
Abstract:
The main purpose of this study was to develop a simple precise, rapid and accurate UV-visible spectrophotometric method for determination of Bumetanide in spiked human urine by extracting the Bumetanide from spiked human urine using ethyl acetate after extraction it was scanned between 200-400nm by using UV detector and its absorbance maxima was found to be 222nm.The calibration curve was linear in the range of 1-17 µg/ml. .the recovery and assay studies of bumetanide were within 93-94.85% indicating that the proposed method can be estimation of bumetanide.
Keywords:
Bumetanide; UV Spectrophotometric; Validation; Method development
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