Evaluation of the effectiveness of SARS-CoV-2 molecular diagnostic tests

Safae El Kochri; Zineb Rhazzar; Maha Ouarab; Mouhssine Hemlali; Hajar Zahafi; Mostafa Elouennass; Elmostafa El Fahime; Lamiae Belayachi; Abdelaziz Benjouad; Nadia Touil; Idriss Lahlou Amine; Khalid Ennibi

doi:10.30574/wjarr.2024.24.2.2331

Evaluation of the effectiveness of SARS-CoV-2 molecular diagnostic tests

Safae El Kochri ^{1, 2, *}, Zineb Rhazzar ^{1, 3}, Maha Ouarab ^{4, 5}, Mouhssine Hemlali ^{4, 5}, Hajar Zahafi ⁶, Mostafa Elouennass ⁷, Elmostafa El Fahime ^{4, 5}, Lamiae Belayachi ⁸, Abdelaziz Benjouad ⁸, Nadia Touil ^{1, 9}, Idriss Lahlou Amine ¹ and Khalid Ennibi ^{1, 3}

¹ Center for Virology, Infectious and Tropical Diseases, Mohammed V Military Teaching Hospital, Rabat, Morocco.

²Department of Microbiology, Faculty of Medicine and Pharmacy, Hassan II University, Casablanca.

³ Immunopathology Research Team (ERIP), Faculty of Medicine and Pharmacy, Mohammed V University of Rabat, Morocco.

⁴ Neurosciences and Neurogenetics Research Team, Faculty of Medicine and Pharmacy, Mohammed V University of Rabat, Morocco.

⁵ Functional Genomics Platform, National Center for Scientific and Technical Research (CNRST), Rabat, Morocco.

⁶Faculty of science, Mohammed V University of Rabat, Morocco.

⁷ Department of Bacteriology, Mohammed V Military Instruction Hospital, Rabat, Morocco.

⁸ Health Sciences Research Center, BioMed Unit, International Faculty of Medicine, International University of Rabat, Sala-Al Jadida, Morocco.

⁹Center for Genomics of Human Pathologies, Faculty of Medicine and Pharmacy, Mohammed V University of Rabat, Morocco.

Research Article

World Journal of Advanced Research and Reviews, 2024, 24(02), 2122–2128

Article DOI: 10.30574/wjarr.2024.24.2.2331

DOI url: https://doi.org/10.30574/wjarr.2024.24.2.2331

Publication history:

Received on 30July 2024; revised on 06 November 2024; accepted on 08 November 2024

Abstract:

Since the emergence of SARS-CoV-2 in late 2019, the virus has spread worldwide and posed a major burden on public health. The rapid and accurate identification of patients infected with the virus by real-time reverse transcription polymerase chain reaction (RT-PCR) is an effective measure to limit the spread of the virus. Many commercial kits have been available, but their efficiencies must be independently evaluated before their use in the laboratory. The objective of this study is to evaluate their effectiveness and stability before proceeding with the diagnosis. Ten SARS-COV-2 molecular diagnostic kits used in the Virology laboratory at the Center for Virology, Infectious and Tropical Diseases (CVMIT) at the Mohammed V Military Instruction Hospital (HMIMV) in Rabat between 2020 and 2024 were evaluated using a quantification standard from viral culture supernatant. The effectiveness of the kits varied from 93% to 168% depending on the manipulators and the target genes.

Keywords:

SARS-CoV-2; COVID-19; RT-PCR; Molecular diagnosis

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Evaluation of the effectiveness of SARS-CoV-2 molecular diagnostic tests

Safae El Kochri 1, 2, *, Zineb Rhazzar 1, 3, Maha Ouarab 4, 5, Mouhssine Hemlali 4, 5, Hajar Zahafi 6, Mostafa Elouennass 7, Elmostafa El Fahime 4, 5, Lamiae Belayachi 8, Abdelaziz Benjouad 8, Nadia Touil 1, 9, Idriss Lahlou Amine 1 and Khalid Ennibi 1, 3

Safae El Kochri ^{1, 2, *}, Zineb Rhazzar ^{1, 3}, Maha Ouarab ^{4, 5}, Mouhssine Hemlali ^{4, 5}, Hajar Zahafi ⁶, Mostafa Elouennass ⁷, Elmostafa El Fahime ^{4, 5}, Lamiae Belayachi ⁸, Abdelaziz Benjouad ⁸, Nadia Touil ^{1, 9}, Idriss Lahlou Amine ¹ and Khalid Ennibi ^{1, 3}