Assessment of environmental condition and drying process of the plants on the concentration of alkaloids and cytotoxicity of traditional Ayahuasca Tea
1 Department of Biodiversity, National Institute of Amazonian Research, Manaus, Amazonas, Brazil.
2 Department of Biological Sciences, Higher School of Agriculture “Luiz de Queiroz”, University of São Paulo, Piracicaba, São Paulo, Brazil.
3 Faculty of Pharmaceutical Sciences, University of Campinas, São Paulo, Brazil.
Research Article
World Journal of Advanced Research and Reviews, 2021, 10(02), 075–089
Article DOI: 10.30574/wjarr.2021.10.2.0209
Publication history:
Received on 03 April 2021; revised on 06 May 2021; accepted on 09 May 2021
Abstract:
Introduction: Ayahuasca is a traditional psychoactive tea of Amazonian indigenous, used medicinal and spiritual purposes. Wide variation in the concentration of N,N-dimethyltryptamine (DMT), Harmaline (HRL), Harmine (HRM) and Tetrahydroharmine (THH) alkaloids in Ayahuasca has been reported worldwide.
Objective: To evaluate the causes of variations in alkaloids concentrations of Ayahuasca prepared with fresh and dehydrated plants from different environments and determine the best drying method to plants according to alkaloids content and cytotoxicity of Ayahuasca tea.
Material and methods: The environment interference on the alkaloids of the two species was evaluated in samples of Ayahuasca tea prepared with fresh plants. The most suitable drying process to the two species was evaluated in sample Ayahuasca tea prepared with plants submitted to drying under the sun conditions and five different temperatures in forced circulation oven. The concentration of the alkaloids determined by high performance liquid chromatography with UV-vis detector with diode array detection (HPLC-DAD). The in vitro cytotoxicity of Ayahuasca was evaluated in human keratinocytes cells (HaCaT) by colorimetric assay.
Results: Environmental characteristics, preparation process and temperature of plants drying interfered on DMT, HRL, HRM and THH concentrations of Ayahuasca. No effect cytotoxicity was detected with relationship to psychoactive alkaloids in samples of Ayahuasca tea prepared with fresh or dried plants.
Conclusion: Concentration of DMT, HRL, HRM and THH alkaloids in Ayahuasca are influenced by plants environmental. The most suitable drying process was obtained in forced circulation oven at 43 and 45°C to P. viridis leaves and B. caapi stems respectively. The Ayahuasca prepared with fresh or dry plants no showed cytotoxicity in human keratinocytes cells.
Keywords:
Traditional Ayahuasca; Alkaloids; Dry Plants; Psychotria; Banisteriopsis; Cytotoxicity.
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