The in vitro antisickling effect of purified alkaloids of Cremaspora triflora (Thonn.) K. Schum. (Rubiaceae) and Macaranga schweinfurthii Pax. (Euphorbiaceae)

Marsi Mbayo Kitambala 1, *, Emery Kalonda Mutombo 1, Blandine Nkiko Niyibizi 1, Grégoire Sangwa Kamulete 2, Glauber Mbayo Kalubandika 2, Jean Mulamba Muidikija 3, Evodie Numbi wa Ilunga 2, Richard Muya Kalunga 2, Serge Mbuyi Kalonji 2, Welcome Muyumba Nonga 3,7, Nathalie Mwenze Musenge 2, James Maloba Mwinesenge 3, Faustin Mwamba Maseho 5, Albert Kanangila Bujitu 1, Pius Mpiana Tshimankinda 6 and Jean-Baptiste Lumbu Simbi 1.

1 Department of Chemistry, Faculty of Sciences, University of Lubumbashi, DR Congo.
2 Faculty of Pharmaceutical Sciences, University of Lubumbashi, DR Congo.
3 Department of Chemistry, Teacher’s Training College of Mbuji-Mayi, Mbuji-Mayi, DR Congo.
4 Department of Chemistry, Teacher's Training College of Lubumbashi, Lubumbashi, DR Congo.
5 Faculty of Medicine, University of Kamina, Kamina, DR Congo.
6 Department of Chemistry, University of Kinshasa, Kinshasa, RD Congo.
7 Department of Therapeutic Chemistry and Pharmacognosy, University of Mons, Belgium.
 
Research Article
World Journal of Advanced Research and Reviews, 2021, 09(003), 129-137
Article DOI: 10.30574/wjarr.2021.9.3.0074
 
Publication history: 
Received on 04 February 2021; revised on 06 March 2021; accepted on 09 March 2021
 
Abstract: 
Introduction and objective: Sickle cell disease is an inherited pathology to an abnormality of hemoglobin precisely hemoglobin S for which there is no curative therapy. It mainly affects sub-Saharan African and Caribbean populations. Thus, this study aims to make the phytochemical screening of Cremaspora triflora and Macaranga schweinfurthii as well as to evaluate the antisickling activity of their purified alkaloids.
Methodology: Chemical screening was performed using color and precipitation tests as well as the foam index method. The extraction of the alkaloids was carried out with organic solvents in a basic medium while the purification by open column chromatography. The evaluation of the antisickling activity was carried out by Emmel’s test.
Results: The chemical screening highlighted alkaloids, steroids, saponins, tannins, and terpenoids in the species studied. Flavonoids and anthocyanins were present in organs of Macaranga schweinfurthii, but absent in Cremaspora triflora. The extraction showed that Macaranga schweinfurthii leaves contained 0.59% alkaloids and 0.73% alkaloids in Cremaspora triflora leaves. The alkaloids purification allowed to obtain an alkaloidal fraction MS1 (1.24 g, 70.05%) from Macaranga schweinfurthii and two fractions [CT2 (0.934 g, 63.97%) and CT3 (0.006 g, 0.41 %)] from Cremaspora triflora which tested positive with Dragendorff and Wagner reagents. The antisickling activity evaluation showed that the SIR varied between 36.00% (0.25 mg/ml) and 90.66% (1 mg/ml) for the alkaloid solutions of Cremaspora triflora (IC50 of 0.51 mg/ml) as well as between 4.00% (0.25 mg/ml) and 33.33% (1 mg/ml) for the alkaloid solutions of Macaranga schweinfurthii (IC50 of 1.40 mg/ml).
Conclusion: This study showed that the purified alkaloids of the studied plant species have an inhibitory power on sickling. 
 
Keywords: 
Chemical screening; alkaloid; Antisickling; Euphorbiaceae; Rubiaceae
 
Full text article in PDF: 
 
Copyright information: 
Copyright © 2021 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0

 

Share this