1 Department of Genetics, Biology and Animal Physiology, Faculty of Agroforestery, Agro valorisation Laboratory, Jean Lorougnon Guédé University, BP 150Daloa, Ivory Coast.
2 Department Animal Biology, Faculty of Biological Sciences, Péléferon Gon Coulibaly University,BP 1328 Korhogo, Ivory Coast.
3 Department Animal Biology, Faculty of Sciences and Technologies, Laboratoire Sciences de Biologie Animale, Alassane Ouattara University, BPV 18 Bouaké 01, Ivory Coast.
4 Department of Applied Biology and Health, Faculty of Biosciences, Biology and Health Laboratory, Félix Houphouët Boigny University, 22 BP 582 Abidjan 22, Ivory Coast.
World Journal of Advanced Research and Reviews, 2026, 30(02), 362-371
Article DOI: 10.30574/wjarr.2026.30.2.1186
Received on 17 March 2026; revised on 02 May 2026; accepted on 05 May 2026
Background: Diabetes mellitus is a disease that causes numerous organ malfunctions in animals.The prohibitive cost of modern medicines for populations in developing countries is directing patients suffering from this pathology towards phytotherapy. A preclinical study of herbal remedies is therefore highly desirable.
Objective: The aim of this study was to evaluate the impact of aqueous extracts of Annona senegalensis (Annonaceae) (EAAs) and Hallea ledermannii (Rubiaceae) (EAHl) on the liver and pancreas of diabetic Wistar rats.
Methodology: Induction of diabetes mellitus in normal rats with body weights ranging from 200 to 250 g was achieved by intraperitoneal injection of alloxane (75 mg / kg bw). After four (4) and thirteen (13) weeks of treatment, some diabetic rats were sacrificed by decapitation following ethyl urethane anesthesia (Bouafou, 2007). The liver and pancreas of these rats were dissected to determine their relative weight, followed by histological analysis.
Results: After four (4) and thirteen (13) weeks of treatment, the relative liver and pancreas weights of all these test animals did not change significantly compared with those of non-diabetic rats. At four (4) weeks of treatment, histological analysis of the liver revealed lesions of diffuse moderate glycogen overload in the liver parenchyma and hepatocyte apoptosis in rats treated with EAAs (100 and 200 mg / kg bw), EAHl (200 and 400 mg / kg bw) and Glibenclamide (10-2 g / kg bw). In the pancreas of test rats, histological studies showed that the islets of Langerhans of diabetic rats treated with plant extracts were necrotic and affected by apoptosis, compared with those of non-diabetic rats. Severe lymphocytic chronic pancreatitis was also observed in this organ of these animals. After 13 weeks (J91) of treatment, histological sections of the pancreas of diabetic rats treated with glibenclamide and our natural substances showed a normal pancreas with islet regeneration comparable to that of normal rats.
Conclusion: This study highlighted the pancreatic regenerative activities of EAAs and EAHl in diabetic rats.
Annona Senegalensis; Hallea Ledermannii; Histology; Liver; Pancreas; Rat; Diabetes
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Gooré Guy Charles Golé NANTI, Bi Lané Andrien GOH, Kouehiouon Justin Anderson-Bel DJIKE and Bi Semi Anthelme NENE. Effects of aqueous extracts of Annona senegalensis and Hallea ledermannii on hepatic and pancreatic histological architecture in diabetic Wistar rats. World Journal of Advanced Research and Reviews, 2026, 30(02), 362-371. Article DOI: https://doi.org/10.30574/wjarr.2026.30.2.1186.