Evaluation of the ameliorative impacts of ethanol rhizomes extract of Curcuma longa on hematological parameters of diabetes- induced male Albinо Wistar rats

The ameliorative effects of ethanol rhizome extract from Curcuma longa on the haematological indicators of male albino Wistar rats (198-225g) induced by alloxan diabetes were assessed.  Thirty healthy animals were randomly divided into six groups of five rats each.  Diabetes mellitus was generated in groups 1 to 5 through the peritoneum. Subsequently, groups 1, 2, and 3 received daily oral administration of 200 mg/kg, 500 mg/kg, and 700 mg/kg of C. longa extract, respectively, for one month.  Group 4 received 500mg/kg of metformin, a typical anti-diabetes medication (positive control), whereas groups 5 and 6 were untreated, serving as negative and normal controls, respectively.  The haematological indices measured included Red Blood Cells (RBCs), White Blood Cells (WBCs), Platelets (PT), Haemoglobin (Hb), Haematocrits (HCT), Mean Corpuscular Haemoglobin Concentration (MCHC), Mean Corpuscular Volume (MCV), Lymphocytes (LYM), Monocytes (MON), Basophils (BAS), and Eosinophils (EOS).  When compared to groups 1, 2, 3, 5, and 6, group 4 had a statistically significant increase (P<0.05) in WBCs and PT.  Compared to groups 1, 2, 5, and 6, group 4 saw a substantial drop (P<0.05) in RBCs and Hb.  When compared to groups 2, 3, and 4, group 1 saw a big rise in HCT (P<0.05).  When compared to groups 3, 5, and 6, group 4 was determined to be significantly lower (P<0.05).  There was no significant difference (P>0.05) between and among groups for MCV and MCH.  There was a big difference in MCHC between group 2 and groups 1 and 3 (P<0.05). In group 1, lymphocytes dropped a lot (P<0.05) compared to groups 2, 3, 5, and 6.  When group 4 was compared to group 2, MON went up a lot (P<0.05). When compared to groups 4 and 6, group 3 saw a big rise in BAS (P<0.05).  In group 3, EOS went raised a lot (P<0.05) compared to groups 4 and 5. These results suggested that diabetes affected the haematological indices in the mice, while ethanol rhizome extract appears to mitigate the consequences.