Effect of aqueous extract of Azadirachta indica on rifampicin- induced jaundice in rabbits
1 Department of Medical Laboratory Sciences, Faculty of Basic Medical Sciences, Bingham University, Karu, Nigeria.
2 Department of Pharmacology & Therapeutics, College of Medicine and Health Sciences, Bingham University, Jos, Nigeria. 3 Department of Pharmacology, Faculty of Pharmaceutical Sciences, University of Jos, Nigeria.
4 Department of Pest management Technology, Federal College of Forestry, Jos, Nigeria.
Research Article
World Journal of Advanced Research and Reviews, 2020, 08(02), 205-212
Article DOI: 10.30574/wjarr.2020.8.2.0422
Publication history:
Received on 13 November 2020; revised on 20 November 2020; accepted on 21 November 2020
Abstract:
High rate of dependence on medicinal plants for maintenance of health care needs over the decades in communities all over the world especially in Africa and Asia needs adequate scientific verification and authentication. Liver related diseases have however become a global problem. Jaundice was established on 9 of a total population of 12 rabbits of mixed sex, weighing 1.50kg - 2.34kg using an overdose pretreatment of Rifampicin (300mg/ kg bw) P.O. After 3 days, the Animals were administered with various doses of aqueous extract of Azadirachta indica (600, 750, 900mg/kg bw) P.O for each group of rats – A, B, C and D (control ), after which the animals were bled via marginal ear vein at zero hour, 7th and 14th days and the blood used for the following assays: Alkaline Phosphatase, Acid Phosphatase, Total Bilirubin, Conjugated Bilirubin and Alanine Transaminase. Experimental animals exhibited lack of appetite, reduced activity, scratching of the body, emesis, yellow coloration of the sclera, light-colored stool and a little dark urine over time. There was also increase in the levels of the biochemical parameters as the day increased. Aqueous extract of Azadirachta indica (Neem) exhibited increasing elevated levels of Alkaline Phosphatase (ALP), Acid Phosphatase (AP), Total Bilirubin (T/Bil), Conjugated Bilirubin (Conj/Bil), and slightly in Alanine Transaminase (ALT), thus exerted elevated hepatotoxicity on Rifampicin-induced Rabbits. Therefore, the traditional use of Azadirachta indica in management of jaundice should be discontinued.
Keywords:
Azadirachta indica; Rabbit; Bilirubin; Jaundice; Rifampicin
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