1 Laboratory of Natural Sciences and Applications (LSNA), Higher Normal School of Natitingou, National University of Sciences, Technologies, Engineering, and Mathematics of Abomey, BP 72 Natitingou, Benin.
2 National Laboratory of Narcotics and Toxicology (LNST)-Benin Center for Scientific Research and Innovation (CBRSI)/UAC, Benin. Campus du Champs de Foire-Faculty of Health Sciences (FSS), 04 BP 1357 Cotonou, Republic of Benin.
3 Teaching and Research Unit in Occupational Health and Environment (URESTE), Department of Public Health (DSP), Faculty of Health Sciences (FSS), University of Abomey-Calavi (UAC), 01 BP188 Cotonou, Republic of Benin.
4 Laboratory of Animal Biotechnology and Meat Technology (LBATV), Animal Health Production Department (PSA)University of Abomey-Calavi (UAC), 0BP 2009 DPSA, Cotonou, Republic of Benin.
5 Laboratory of Biology and Molecular Typing in Microbiology; Department of Biochemistry and Cellular Biology, Faculty of Science and Technology, University of Abomey-Calavi (UAC), 05 BP 1604 Cotonou, Republic of Benin.
6 Unit of Environmental Chemistry and Interactions on Living Things (UCEIV), University of Littoral Côte d’Opale (ULCO), 189A avenue Maurice Schumann, 59140 Dunkirk, France.
7 Laboratory of Biochemistry and Molecular Biology, Faculty of Science and Technology (FAST), University of Abomey-Calavi, 04 BP 0320, Cotonou, Benin.
Research Article
World Journal of Advanced Research and Reviews, 2024, 23(01), 1253-1264
Received on 01 June 2024; revised on 12 July 2024; accepted on 15 July 2024
Objective. This study aimed to explore the "In Vitro" antimicrobial activity of extracts of leafy stems of M. charantia on a few multi-resistant germs.
Methods: Phytochemical screening of M. charantia leafy stem powder was carried out by the methods of colorimetry and thin layer chromatography followed by the search for larval cytotoxicity. The sensitivity test by the solid medium diffusion method and the search for resistance genes were carried out on E. coli ATCC25922 then on K. pneumoniae, K. oxytoca, and E. coli isolated from hospital samples. Flavonoids, alkaloids, stetol-terpenes and saponosides were identified in the powder of the leafy stems of M. charantia.
Results: No cytotoxic effects were observed in Artemia salina at the LC 50 of 6.25 mg/ml. With the exception of Ciprofloxacin, Ertapenem and Ceftriaxone which showed respective resistance rates of 60%, 90% and 90%, absolute resistance, i.e. 100%, was observed against Ampicillin, Aztreonam, and Augmentin. The resistance genes present in the bacterial strains studied were SHV, TEM, CTX-M1 and CTX-M15. The sensitivity tests carried out indicate that the aqueous and ethanolic extracts were active on the strains tested with respectively average inhibition diameters of between 9 ±1 and 14 ±1 mM then between 9 ±1 and 12 ±1 mM.
Conclusion: This study revealed antimicrobial activity of each of the aqueous and ethanolic extracts of the leafy stems of M. charantia of the multidrug-resistant bacterial strains studied.
M. charantia; Resistance genes; Sensitivity test; Antimicrobial activity; Bacterial multi-resistance
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Ténor Dias-Mendel ALLODE, Ferdinand Mènakpo ADOUNKPE, Honesty TOHON, Viridiane Newlyne Jesuklo AHOLOUKPE, Saliou LATOUNDJI, Nathalie Gbessiwèdè HOUNMASSE, Akodji Dèfognon Fiacre MIGAN, Issiaka Karim YOUSSAO and Lamine Saïd BABA MOU. "In vitro” antimicrobial activity of extracts from the leafy stems of Momordica charantia Linné (Cucurbitaceae) on some multi-resistant microbial strains. World Journal of Advanced Research and Reviews, 2024, 23(1), 1253-1264. Article DOI: https://doi.org/10.30574/wjarr.2024.23.1.2079