In vitro anticancer and antiproliferative activity of crocin on HCT-116 cells
1 Department of Biochemistry Faculty of Veterinary, Ataturk University, Erzurum, Turkey.
2 Demirozu Vocational School, Bayburt University, Bayburt, Turkey.
Research Article
World Journal of Advanced Research and Reviews, 2022, 15(03), 290–297
Publication history:
Received on 13 August 2022; revised on 18 September 2022; accepted on 20 September 2022
Abstract:
One of the world’s main causes of cancer-related fatalities worldwide is colorectal cancer. The adherent epithelial cells known as HCT116 (human colorectal carcinoma) are frequently employed to examine inflammatory responses in colonic epithelial cells. They are derived from the human colorectal cancer cell line. Crocin, a potent antioxidant, anticarcinogenic was tested in this study to see how it affected the HCT-116 human colorectal cancer cell line. MTT analysis was used to assess cell viability. Crocin's antiproliferative activity to inhibit the proliferation of HCT 116 cancer cells was examined. Crocin displayed the outstanding activities for MTT test, IC50= 10.57 μL/mL for 24 hours was calculated as IC50= 3.29 μL/mL for 48 hours on HCT 116 cell line. Total antioxidant status (TAS) and total oxidant status (TOS) in the prepared cell lysate were determined by using commercial kits. Crocin is thought to be a high potential agent to be used in treatment.
Keywords:
Colorectal cancer; Crocin; HCT 116; MTT
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