1 Department of Chemistry, Government Degree College, Seethanagaram, Andhra Pradesh, India.
2 Department of Pharmacy, Aditya College of Pharmacy, Surampalem, Andhra Pradesh, India.
Review Article
World Journal of Advanced Research and Reviews, 2023, 18(02), 492-501
Article DOI: 10.30574/wjarr.2023.18.2.0851
Received on 08 March 2023; revised on 09 May 2023; accepted on 11 May 2023
This review article discusses the impact of nanostructured stationary phases on liquid chromatography and separation science. These materials have revolutionized chromatography by enabling unprecedented levels of sensitivity, resolution, and applicability. Nanoporous silica, graphenic, monolithic, and nanoparticle-based phases continue to push the boundaries of biomolecular analysis, molecular diagnostics, and traceability testing. Nanostructured phases have made early detection of diseases, comprehensive profiling of proteomes, enhanced food origin traceability, and sensitive environmental monitoring possible. They facilitate isolation and analysis of biomacromolecules, extracellular vesicles, viruses, and trace constituents with high specificity and sensitivity even from minimal sample volumes. Furthermore, nanostructured phases are enabling integrated techniques, sensing capabilities, and responsive microdomains for advanced detection, purification, and separation of analytes. Continued progress in nanomaterial design, surface engineering, and micro-nanofabrication will lead to more sophisticated nano-LC approaches with translation across healthcare, food safety, materials analysis, and global sustainability. The review concludes that nanostructured stationary phases represent a pivotal frontier in chromatography and analytical sciences with tremendous potential to transform molecular diagnosis, precision medicine, origin traceability, and monitoring of health, food, and environment quality. Nano-LC promises to make comprehensive and minimally invasive molecular-level understanding more feasible, accessible, and impactful. These materials are an enabling technology with immense and far-reaching possibilities that will likely shape developments in analytical sciences and their use for years to come.
Liquid Chromatography; Nanoparticles; Silica Gel; Proteomics; Molecular diagnostics
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Sravani Ratnam Arji, Sarma SRS Eranki, Suryasree Pecchetty and Prakash Nathaniel Kumar Sarella. Unconventional stationary phases: Nanomaterials, nanoparticles and the future of liquid chromatography. World Journal of Advanced Research and Reviews, 2023, 18(2), 492-501. Article DOI: https://doi.org/10.30574/wjarr.2023.18.2.0851