Thermodynamics of enzymatic hydrolysis of cellulose
Designer Energy Ltd, 2 Bergman Str., Rehovot 7670504, Israel.
Research Article
World Journal of Advanced Research and Reviews, 2024, 21(02), 577–586
Article DOI: 10.30574/wjarr.2024.21.2.0458
Publication history:
Received on 28 December 2023; revised on 04 February 2024; accepted on 07 February 2024
Abstract:
In this research, the thermodynamical analysis of the enzymatic hydrolysis of cellulose samples with different crystallinity has been performed. Calorimetric methods were used to determine the combustion enthalpies of cellulose samples and enthalpies their interaction with water. As a result, the standard formation enthalpies of dry and wet cellulose samples as well as of glucose solutions were calculated. In addition, also the standard entropies were found. Based on the obtained parameters, the thermodynamic functions of the hydrolysis reaction at the standard temperature (298 K) were calculated. It was established that the hydrolysis process of semi-crystalline cellulose samples is exothermic. Since reaction enthalpy is negative and the temperature-entropy factor is positive, the Gibbs potential of this process becomes negative, which contributes to the implementation of enzymatic hydrolysis of cellulose at standard temperature. Moderate enhancement in temperature to optimal value (323 K) increases the negative Gibbs potential and thereby promotes the hydrolysis of cellulose substrates. A correlation was found between the negative value of the Gibbs potential and the concentration of the resulting glucose solutions. It was also shown that the enzymatic hydrolysis of completely crystalline cellulose cannot be performed even at optimal temperature due to the zero value of the Gibbs potential.
Keywords:
Cellulose; Crystallinity; Hydrolysis; Glucose; Enthalpy; Entropy; Gibbs potential; Thermodynamic analysis
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