Department of Biochemistry, Laboratory of Pharmacology and Toxicology, Faculty of Science, University of Yaoundé I, 812 Yaoundé, Cameroon.
Research Article
World Journal of Advanced Research and Reviews, 2023, 18(01), 781-795
Article DOI: 10.30574/wjarr.2023.18.2.0854
Received on 02 April 2023; revised on 14 May 2023; accepted on 17 May 2023
Background: Wild edible fruits are good source of phenolic compounds such as anthocyanins. Those compounds are known for their anti-inflammatory potential. They interact with many mediators of the physiopathological pathway such as NF-kB which is at a central position in the inflammatory response in macrophages. This study aimed to determine the anti-inflammatory and antioxidant potential of ten wild edible fruits aqueous extracts.
Methods: The antioxidant potential was evaluate through the DPPH, ABTS and OH radical scavenging activity; the ferric reducing agent power and the total antioxidant capacity assessment. Also, the total phenolic and flavonoids, anthocyanins compounds and vitamin C content were assayed. For the anti-inflammatory activity, a primary macrophage culture was used. Inflammatory mediators such as nitric oxide (NO), alkaline phosphatase, NADPH oxidase and 5-lipoxigenase activity was assayed.
Results: Alchornea cordifolia and Pentaclethra macrophylla extracts showed the greatest antioxidant activity respectively (Antiradical power (AP)=5.82 mol of DPPH/mg of extract and AP=5.05 mol of DPPH/mg of extract for DPPH scavenging assay); (IC50=1.62µg/ml and IC50=1.60µg/ml for ABTS radical scavenging assay); (IC50=32.67µg/ml and IC50=70.01µg/ml for OH radical scavenging assay) and (EC50=54.06µg/ml and EC50=17.46µg/ml for ferric reducing antioxidant power) with a non-significant difference with the reference compound. For the anti-inflammatory activity, firstly it has been observed that Alchornea cordifolia’s extract exhibits the greatest potential against NADPH oxidase activity inhibition (IC50=36.20µg/ml); alkaline phosphatase activation (EC50=0.27µg/ml) with non-significant difference with the reference compound. Secondly, Pentaclethra macrophylla’s extract demonstrated a high ability to inhibit NO production (IC50=5.79µg/ml) and 5-lipoxigenase (IC50=1.41µg/ml).
Conclusion: From the results obtained the assessed wild fruit extracts appears to be good sources for anti-inflammatory and antioxidant bioactive molecules. They could be beneficial for the research of new modulators for the management of inflammatory disorders.
Wild fruits; Anti-inflammatory; Antioxidant; Cameroon
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Brice Edie Enang II, Sylvain Nsangou Pechangou, Frederic Nico Njayou, Marie Louise Medoua and Paul Fewou Moundipa. Screening of ten wild fruits extracts from Cameroon’s flora for their anti-inflammatory and antioxidant potential. World Journal of Advanced Research and Reviews, 2023, 18(2), 781-795. Article DOI: https://doi.org/10.30574/wjarr.2023.18.2.0854