1 Department of Microbiology and Immunology, College of Graduate Studies, Midwestern University, Downers Grove, IL, USA.
2 Department of Chemistry, American University, Washington, D.C., USA.
Research Article
World Journal of Advanced Research and Reviews, 2022, 14(02), 603–612
Received on 19 April 2022; revised on 22 May 2022; accepted on 25 May 2022
Tumor interiors undergo prolonged anoxia; however, the pathways involved have not been identified. Since NO and H2S function in prokaryotic anaerobic respiration, the effect their pathway elements have on HeLa 229 cell viability was measured after 10 days anaerobic incubation. Arginine or xanthine (NO pathway precursors) increased cell viability (13.1- and 4.4-fold, respectively). The H2S pathway precursor, cysteine, also enhanced viability (9.8-fold), as did H2S donor GYY4137, or inhibitor of glutathione synthesis, propargylglycine, (40- and 85-fold, respectively). These results demonstrate that cell viability after extended anaerobic incubation (10 days) can be modulated by affecting NO or H2S pathways.
Cysteine; Hydrogen Sulfide; Arginine; Xanthine; Nitric Oxide; Hypoxia Inducible Factor (HIF); Anoxia
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Balbina J Plotkin, Ira M Sigar, Amber Kaminski and Monika I Konaklieva. Putative pathways fueling anaerobic mitochondrial respiration. World Journal of Advanced Research and Reviews, 2022, 14(2), 603-612. Article DOI: https://doi.org/10.30574/wjarr.2022.14.2.0464