Protective effect of Juglans regia L. (walnut) leaves extract against indomethacin induced gastric mucosal damage in rats
Department of Physiology, University of Erzincan Binali Yıldırım, Faculty of Medicine, Erzincan – Turkey.
Research Article
World Journal of Advanced Research and Reviews, 2022, 13(03), 264–270
Article DOI: 10.30574/wjarr.2022.13.3.0230
Publication history:
Received on 07 February 2022; revised on 10 March 2022; accepted on 12 March 2022
Abstract:
Indomethacin, an anti-inflammatory agent used for pain, fever and inflammation, causes severe lesions in the gastrointestinal tract in humans and animals. For this reason, there has been an increase in the search for new compounds that can help prevent and/or alleviate these lesions caused by indomethacin. The aim of the present study is to evaluate the gastroprotective effect of Juglans regia administration against indomethacin induced gastric lesion. A total of forty rats were divided equally among four groups for the experiments. Groups were designed as Control (C), Indomethacin (IND), Juglans regia + Indomethacin (JR+IND), and Pantoprazole + Indomethacin (PAN+IND). Following the 7-day adaptation period, substance applications (pantoprazol and Juglans regia extract) were made for 10 days. In the IND, JR+IND and PAN+IND groups were administered indomethacin 100 mg/kg single dose on day 11th. Indomethacin caused significant increases in the stomach IL-6 and TNF-α levels compared with the C group (respectively, p<0.001, p<0.01). In the PAN+IND and JR+IND groups showed a significant lower in the IL-6 levels compared with IND group (respectively, p<0.001, p<0.01). The C-reactive protein (CRP) and Cyclo-oxygenase-2R (COX-2R) levels of IND group were increased compared to C group (p<0.001). There was a significant decrease in CRP and COX-2R levels after PAN and JR treatment compared with IND (p<0.001). COX-2, CRP and proinflammatory cytokines is seen as the main target for the treatment of inflammatory diseases. As a result; it was determined that JR has a protective effect, which may be related to decrease inflammatory markers, against of gastric damage caused by indomethacin.
Keywords:
Gastric ulcer; Juglans regia; Indomethacin; C-reactive protein; Cyclo-oxygenase-2R
Full text article in PDF:
Copyright information:
Copyright © 2022 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0