Prevalence of metallo-beta-lactamase in clinical isolates of Pseudomonas aeruginosa and Proteus mirabilis in Benin City, Nigeria
1 Department of Medical Laboratory Science, University of Benin, Benin City, Edo state, Nigeria.
2 Department of Medicine and Surgery, Obafemi Awolowo University, Ile-Ife, Osun State, Nigeria.
3 Department of Public Health Biotechnology, University of Ibadan, Ibadan, Oyo State, Nigeria.
4 Department of Chemistry, University of Ilorin, Ilorin, Kwara State, Nigeria.
5 Department of Biochemistry, Yaba College of Technology Yaba, Lagos state Nigeria.
6 Department of Microbiology, University of Nigeria, Nsukka, Enugu State, Nigeria.
Research Article
World Journal of Advanced Research and Reviews, 2024, 23(01), 770–774
Publication history:
Received on 26 May 2024; revised on 07 July 2024; accepted on 09 July 2024
Abstract:
Carbapenems are the prime choice of treatment for severe cases of infections caused by Multi-Drug-Resistant Pseudomonas aeruginosa and Proteus mirabilis. Nevertheless, Metallo-Beta-Lactamase (MBL) production by these organisms has led to carbapenem resistance which is a global threat. This study aimed to determine the prevalence and antimicrobial susceptibility profile of MBL in clinical isolates of Pseudomonas aeruginosa and Proteus mirabilis in Benin City, Nigeria. 354 non monotonous clinical isolates of Pseudomonas aeruginosa (282) and Proteus mirabilis (72) used were obtained from various clinical samples from tertiary hospitals in Benin City. Identification of these isolates were done using standard microbiological techniques. Antimicrobial susceptibility test was performed using Kirby-Bauer disk diffusion method. MBL production was detected using Imipenem Ethylene-Diamine-Tetra-Acetic Acid combined disc test method. Of the total 354 clinical isolates tested, 115 (32.48%) were MBL and the prevalence of this resistant isolates was significantly higher in Pseudomonas aeruginosa (46.8%) compared to Proteus mirabilis (P=0.0001). Among the metallo-beta-lactamase producing isolates of Pseudomonas aeruginosa higher prevalence were reported from Pleural fluid and cerebrospinal fluid samples with 6 (100%) and 3 (100%) respectively. This difference was statistically significant (P=0.0001). Susceptibility testing showed that isolates that produced beta-lactamase demonstrated poorly against cephalosporin, amoxicillin-clavulanate, gentamicin and floroquinones than non-beta- lactamase producers. A prevalence of 46.8% was reported for MBL producing Pseudomonas aeruginosa and 12.5% was reported for MBL producing Proteus mirabilis. Isolates that produced the MBL enzyme were more resistant to antibacterial agents. Measures to control and curb the spread of MBL producing clinical isolates are highly advocated
Keywords:
Prevalence; Multi-Drug Resistance; Carbapenems; Metallo-Beta-Lactamase; Pseudomonas Aeruginosa; Proteus Mirabilis
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