New genomic regions for resistance to anthracnose (Colletotrichum lindemuthianum) through GBS-based genome-wide association study in common bean (Phaseolus vulgaris)
1 Universidade Estadual de Maringá, Departamento de Agronomia, Maringá, Brazil.
2 Instituto Agronômico de Pernambuco, Recife, Brazil.
3 University of California, Department of Plant Sciences / MS1, Section of Crop & Ecosystem Sciences, Davis, USA.
Research Article
World Journal of Advanced Research and Reviews, 2021, 12(01), 020–040
Article DOI: 10.30574/wjarr.2021.12.1.0493
Publication history:
Received on 24 August 2021; revised on 28 September 2021; accepted on 30 September 2021
Abstract:
The most effective strategy to manage bean anthracnose (ANT), caused by Colletotrichum lindemuthianum, is the use of resistant cultivars. This study aimed to evaluate resistance reactions of common bean accessions to C. lindemuthianum races 2, 9 and 1545, and to perform genome-wide association study (GWAS). Hence, 89 accessions were phenotyped and genotyped through genotyping by sequencing (GBS). As a result, 48 accessions resistant to all evaluated races were identified. Moreover, single-nucleotide polymorphisms (SNP) significantly associated with resistance were identified in new regions of chromosomes Pv03, Pv05 and Pv06, and also at the beginning of Pv04 and end of Pv11, where other resistance genes have been previously found. In reference genome these regions contain model genes encoding resistance proteins as kinases, leucine-rich repeats, receptor-like protein, copper transport protein, pentatricopeptide repeats, calcium-dependent protein kinases, and ethylene-responsive transcription factors. The genomic regions associated to ANT resistance found in this study should be validated for further use in marker assisted selection and gene pyramiding. Together with new sources of ANT resistance our findings show promise for further crop improvement.
Keywords:
GWAS; GBS; Colletotrichum lindemuthianum; Anthracnose; Resistance sources; Candidate genes
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Copyright © 2021 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0