Nescafé coffee caused changes in hematological parameters of alloxan-induced diabetic Wistar rats

Nonso Odikpo 1, Francis Oguwike 1, Cornelius Nwozor 1, *, Onyekachukwu Oguekwe 1, Kosisochukwu Ifemenam 2, Henry Enwelum 2 and Okechukwu Peter Nwabuokei 3

1 Department of Physiology, Faculty of Basic Medical Sciences, Chukwuemeka Odumegwu Ojukwu University, Uli campus, Anambra State, Nigeria.
2 Department of Obstetrics and Gynecology, Faculty of Clinical Sciences, Chukwuemeka Odumegwu Ojukwu University Teaching Hospital, Amaku, Awka, Anambra State, Nigeria.
3 Department of Medicine and Surgery, Faculty of Medical Sciences, International University of Health Sciences, St. Kitts and Nevis State, West Indies.
Research Article
World Journal of Advanced Research and Reviews, 2024, 22(03), 923–930
Article DOI10.30574/wjarr.2024.22.3.1794
Publication history: 
Received on 05 May 2024; revised on 11 June 2024; accepted on 14 June 2024
This study investigated the effect of Nescafe coffee on the haematological parameters in alloxan-induced diabetic male Wistar rats. Thirty male Wistar rats of body weight between 150 and 260 g were used for the study. They were divided randomly into five groups of six rats each. The 24 rats in groups 2, 3, 4, and 5 were alloxanized with 120 mg/kg of alloxan (intraperitoneally) and were confirmed diabetic before the commencement of the experiment. Group 1 rats (control) received feed and water, group 2 (diabetic rats) received 1.5 ml of 0.9% normal saline, group 3 received 500 mg/kg of Nescafe coffee extract, group 4 received 800 mg/kg of Nescafe coffee extract, while group 5 received 5 mg/kg of glibenclamide. After 28 days of experiment, the animals were sacrificed and blood samples were collected from the rats via cardiac puncture for haematological tests. Fasting blood glucose level was determined weekly via tail puncture using a glucometer and readings were recorded. The result for RBC showed a significant decrease (P < 0.05) in groups 2 and 4 compared to group 5. PCV result showed a significant decrease (P < 0.05) in group 4 compared to group 5. The MCH and MCHC result showed a significant increase (P < 0.001) in group 3, compared with group 5.There was also a significant decrease (P < 0.001) in group 2, group 4 and group 5, compared with group 1 (control). The haemoglobin, MCV, WBC count, lymphocyte, neutrophil, monocyte counts, MPV, and ESR, result showed no significant difference (P > 0.05) in all other groups when compared with group 1 and group 5. There was a significant decrease (P < 0.05) in the platelet count of the animals in groups 2 and 4 when compared with the control group. Furthermore, a significant decrease (P < 0.001) was noted in the eosinophil count and basophil count (P < 0.01) of the animals in groups 3, 4, and 5 when compared to the control group. On the contrary, a significant increase (P < 0.001) was noted in the eosinophil count and basophil count (P < 0.01) of the animals in group 2 when compared to group 5. In conclusion, the results showed that Nescafe coffee (especially when consumed in high quantity) significantly decreased some of the blood parameters of the diabetic animals.
Nescafe coffee; Haemoglobin; Alloxan-induced diabetes; Wistar rats
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