Loss of function phenotype in chicken embryonic development using the small interfering RNA method
1 Department of Biology, Laboratory of Biochemistry, Faculty of Sciences, Gamal Abdel Nasser University of Conakry (UGANC), BP: 1147 Conakry, Republic of Guinea.
2 School of Life Science, Laboratory of biochemistry, University of Xiamen, Fujian, People's Republic of China. PO Box: 361005.
Research Article
World Journal of Advanced Research and Reviews, 2022, 16(03), 648-654
Article DOI: 10.30574/wjarr.2022.16.3.1383
Publication history:
Received on 07 November 2022; revised on 19 December 2022; accepted on 21 December 2022
Abstract:
Introduction: The present study was based on the microinjection of lentiviruses into chicken embryos in the laboratory to demonstrate loss function phenotype in chicken embryonic development.
Method: This study aims primarily to demonstrate that small interfering RNAs can act effectively on chicken embryonic development by decreasing function of the chicken sphingosine 1 phosphate receptor 1/endothelial differentiation gene 1 (S1P1/EDG-1) during embryogenesis. In addition, chicken embryo fibroblasts (CEF) were prepared, plasmid gene 3132/PLL3.7 was used with 2XHBS for transfection, virus titer was determined prior to virus microinjection into chicken embryos to see the loss-of-function phenotype.
Results: The results obtained after viral microinjection showed that the function of S1P1/EDG-1 gene was progressively reduced from the second day of egg incubation until the fourth day. After the fifth day of incubation, loss of gene function was more marked by bleeding into the embryo sac.
Conclusion: Results from previous studies on other vertebrates such as mice have showed similar results [1, 2].
Keywords:
Microinjection; Embryos; Phenotypes; Small interfering RNA; Transfection; S1p1/edg-1; Embryo fibroblasts
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