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eISSN: 2582-8185 || CODEN: WJARAI || Impact Factor 8.2 ||  CrossRef DOI

Research and review articles are invited for publication in March 2026 (Volume 29, Issue 3) Submit manuscript

Isolation and identification of bacterial contamination from used pens by using nutrient agar medium

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  • Isolation and identification of bacterial contamination from used pens by using nutrient agar medium

Sundarapandi. G 1, *, Midhun P 2, Rithanyaa B 2, Santhosh Kumar S 2 and Senthil kumaran S 2

1 Department of Science, Sathyam International School, Vellakoil, Tiruppur, Tamil Nadu, India.
2 Sathyam International School, Vellakoil, Tiruppur, Tamil Nadu, India.
 
Research Article
World Journal of Advanced Research and Reviews, 2023, 19(03), 675–678
Article DOI: 10.30574/wjarr.2023.19.3.1857
DOI url: https://doi.org/10.30574/wjarr.2023.19.3.1857
 
Received on 11 July 2023; revised on 12 September 2023; accepted on 14 September 2023
 
The pen is a common writing instrument made of plastic or metal with a pointed tip used to apply ink to a surface.  Placing pen on dirty surfaces like other objects exposes it to contamination. In this study was conducted to isolate and identify the bacterial contamination of student’s pen in Sathyam International School, vellakoil, Tiruppur, Tamil Nadu, India by using nutrient agar medium. Samples were prepared using swabs moistened with sterile saline and streaked on nutrient agar plates. Out of 25 samples, 19 samples are contaminated by bacteria such as Escherichia coli and Staphylococcus aureus. Escherichia coli having the highest amount and followed by Staphylococcus aureus having lowest amount colony forming capacity.
 
Pen; Bacteria; Contamination; Nutrient; Pathogen
 
https://wjarr.com/sites/default/files/fulltext_pdf/WJARR-2023-1857.pdf

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Sundarapandi. G, Midhun P, Rithanyaa B, Santhosh Kumar S and Senthil kumaran S. Isolation and identification of bacterial contamination from used pens by using nutrient agar medium. World Journal of Advanced Research and Reviews, 2023, 19(3), 675-678. Article DOI: https://doi.org/10.30574/wjarr.2023.19.3.1857

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