An investigation on the antimicrobial activity of shrimp exoskeleton extracted glycosaminoglycans (GAGs) against Escherichia coli and Staphylococcus aureus
Department of Advanced Zoology and Biotechnology, Guru Nanak College, Chennai- 600042, Tamil Nadu, India.
Research Article
World Journal of Advanced Research and Reviews, 2023, 17(02), 570–577
Publication history:
Received on 04 January 2023; revised on 12 February 2023; accepted on 15 February 2023
Abstract:
The present study was aimed to extract the Glycosaminoglycans (GAGs) from the shrimp head waste and carry out analysis for any antimicrobial activity against Escherichia coli and Staphylococcus aureus. Glycosaminoglycans (GAGs) are linear acidic polysaccharides with repeating disaccharide units. GAGs are important for cell signaling, which controls a wide range of metabolic activities including regulation of cell growth, promotion of cell adhesion, wound repair, proliferation, anti-coagulation and antimicrobial activity. In the present study, the shrimp heads were washed, dried and pulverized. From this pulverized shrimp head waste, extraction of GAG protein was achieved. The total protein content in the biological sample was estimated. Antimicrobial study of GAGs complex was accomplished by using Minimal Inhibitory Concentration, Minimal Bactericidal Concentration, Well Diffusion Method against E. coli and Staphylococcus aureus. It was observed that Staphylococcus aureus has the highest antibacterial activity when compared to Escherichia coli.
Keywords:
Glycosaminoglycans; Minimal Inhibitory Concentration; Minimal Bactericidal Concentration; Well Diffusion Method
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