An insight into the active sites of the catalytic basic protein subunit PB1 of the RNA polymerase of human influenza viruses
Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Madurai –625 021, India.
Research Article
World Journal of Advanced Research and Reviews, 2023, 17(01), 625-656
Article DOI: 10.30574/wjarr.2023.17.1.0109
Publication history:
Received on 09 December 2022; revised on 17 January 2023; accepted on 20 January 2023
Abstract:
RNA polymerase from human influenza viruses is a heterotrimeric enzyme and performs the crucial functions of both transcription and replication for multiplication of the viruses in human cells. The heterotrimeric enzyme is made up of two basic protein subunits (PB1 and PB2) and an acidic protein subunit (PA). All the three subunits perform well-defined function(s) in the transcription and replication processes in the human cells. The basic protein subunit PB1 is shown to possess the polymerization activity, whereas the PB2 and the PA subunits are found to be involved in the cap-snatching and proofreading (PR) activities, respectively. The polymerase activity in the catalytic subunit, PB1, is found to be an RNA-dependent RNA polymerase (RdRp). Multiple sequence alignment (MSA) analysis of the PB1 subunits from all the three human influenza viruses, A, B and C shows large number of highly conserved peptides, amino acid motifs and invariant amino acids. Site-directed mutagenesis (SDM) analysis and X-ray crystallographic data have shown that two completely conserved motifs, viz. –GDN- and –SDD-, are involved in binding to the catalytic Mg2+ ion. These data are in close agreement with the MSA analysis data of the polymerases from all the three human influenza viruses. Furthermore, two highly conserved polymerase catalytic regions are identified in the PB1 subunits by sequence similarity to other DNA/RNA polymerases and hence, are proposed to function in the nucleotidyl transfer activities. Presence of the two catalytic regions suggest that the polymerase may function in a dual mode, i.e., in phase I, in association with the cap-snatching subunit PB2, it could be involved in the synthesis of mRNAs (transcription mode) and once enough proteins are made from the mRNAs, in the second phase, in association with PR exonuclease subunit PA, it could switch to the replication mode to synthesize error-free, exact copies of the viral genome. For both the activities, it could use the same invariant catalytic Mg2+-binding –GDN- and –SDD- motifs.
Keywords:
Human Influenza viruses; RNA polymerase; Polymerase Basic Protein subunit PB1; Catalytic Metal-binding motifs; Polymerase active sites; Mechanism of action
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