IgE, IgA, IgD antibodies detection in human cysticercosis by ELISA test in Madagascar

Prisca Annick RAMANDANIRAINY; Vololoniaina Roseline RAMAROSON; Zara RAZAFIARIMANGA; Julien RAZAFIMAHEFA; Ronan JAMBOU; Voahangy ANDRIANARANJAKA; Abel ANDRIANTSIMAHAVANDY

doi:10.30574/wjarr.2025.27.2.3005

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IgE, IgA, IgD antibodies detection in human cysticercosis by ELISA test in Madagascar

Prisca Annick RAMANDANIRAINY ^{1, 2, *}, Vololoniaina Roseline RAMAROSON ^{2, 3}, Zara RAZAFIARIMANGA ^{2, 3}, Julien RAZAFIMAHEFA ⁴, Ronan JAMBOU ⁵, Voahangy ANDRIANARANJAKA ^{2, 3} and Abel ANDRIANTSIMAHAVANDY ^{2, 3}

¹ Charles Mérieux Infectious Disease Center (CICM), Faculty of Medicine, University of Antananarivo, P.O. Box 906, Antananarivo 101, Madagascar.

² Life and Environment Sciences Doctoral School (SVE), University of Antananarivo, P.O. Box 906, Antananarivo 101, Madagascar.

³ Fundamental and Applied Biochemistry Department (DBFA), Faculty of Sciences, University of Antananarivo, P.O. Box 906, Antananarivo 101, Madagascar.

⁴ Neurology Department, Joseph Raseta Befelatanana Hospital, Antananarivo, Madagascar.

⁵ Immunology Unit, Pasteur Institute of Côte d’Ivoire.

Research Article

World Journal of Advanced Research and Reviews, 2025, 27(02), 1591-1597

Article DOI: 10.30574/wjarr.2025.27.2.3005

DOI url: https://doi.org/10.30574/wjarr.2025.27.2.3005

Publication history

Received on 10 July 2025; revised on 17 August 2025; accepted on 19 August 2025

Abstract

Cysticercosis serological diagnosis available in Madagascar is only based on the IgG detection. Thus, we have planned to perform biological tests in order to detect different human antibodies isotypes (IgE, IgA, IgD) directed against Cysticercus cellulosae and to evaluate the performance of enzyme-linked immunosorbent assay (ELISA).

CS-50 antigen, extracted from Cysticercus cellulosae was used to quantify the rates of these isotypes in the serum and in the cerebrospinal fluid (CSF). After standardizing protocols, 80 patients with neurocysticercosis symptoms from the Neurology Department, Joseph Raseta Hospital Befelatanana, Antananarivo were analyzed.

Results showed high rate of IgD in the serum and high rate of IgA in the CSF. For each studied isotype Ig, ELISA test performance was low compared to ELISA-IgG test as well in the serum than in the CSF.

Keywords

Cysticercus cellulosae; cysticercosis; ELISA; IgE; IgA; IgD.

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Prisca Annick RAMANDANIRAINY, Vololoniaina Roseline RAMAROSON, Zara RAZAFIARIMANGA, Julien RAZAFIMAHEFA, Ronan JAMBOU, Voahangy ANDRIANARANJAKA and Abel ANDRIANTSIMAHAVANDY. IgE, IgA, IgD antibodies detection in human cysticercosis by ELISA test in Madagascar. World Journal of Advanced Research and Reviews, 2025, 27(2), 1591-1597. Article DOI: https://doi.org/10.30574/wjarr.2025.27.2.3005

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IgE, IgA, IgD antibodies detection in human cysticercosis by ELISA test in Madagascar

Prisca Annick RAMANDANIRAINY ^{1, 2, *}, Vololoniaina Roseline RAMAROSON ^{2, 3}, Zara RAZAFIARIMANGA ^{2, 3}, Julien RAZAFIMAHEFA ⁴, Ronan JAMBOU ⁵, Voahangy ANDRIANARANJAKA ^{2, 3} and Abel ANDRIANTSIMAHAVANDY ^{2, 3}

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IgE, IgA, IgD antibodies detection in human cysticercosis by ELISA test in Madagascar

Prisca Annick RAMANDANIRAINY 1, 2, *, Vololoniaina Roseline RAMAROSON 2, 3, Zara RAZAFIARIMANGA 2, 3, Julien RAZAFIMAHEFA 4, Ronan JAMBOU 5, Voahangy ANDRIANARANJAKA 2, 3 and Abel ANDRIANTSIMAHAVANDY 2, 3

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Prisca Annick RAMANDANIRAINY ^{1, 2, *}, Vololoniaina Roseline RAMAROSON ^{2, 3}, Zara RAZAFIARIMANGA ^{2, 3}, Julien RAZAFIMAHEFA ⁴, Ronan JAMBOU ⁵, Voahangy ANDRIANARANJAKA ^{2, 3} and Abel ANDRIANTSIMAHAVANDY ^{2, 3}