Identification of MMP-like protein from Streptococcus mitis
1 Department of Endodontics
2 Bacteriology Osaka Dental University 8-1 Kuzuhahanazonocho Hirakata, Osaka 573-1121, Japan.
Research Article
World Journal of Advanced Research and Reviews, 2020, 08(03), 154-161
Article DOI: 10.30574/wjarr.2020.8.3.0473
Publication history:
Received on 04 December 2020; revised on 11 December 2020; accepted on 13 December 2020
Abstract:
Streptococcus mitis is a normal commensal of the human oral cavity and oropharynx. This microorganism is an opportunistic pathogen in immune compromised hosts and a cause of invasive diseases such as infective endocarditis. We isolated a matrix metalloprotease (MMP)-like protein of S. mitis. The gene for the MMP-like protein was found on the genome sequence database of S. mitis . The gene encodes a protein consisting of 240 amino acid residues with a conserved zinc-binding motif (HEXXHXXGXXH) among the matrix metallopeptidase family. The gene was PCR-amplified from S. mitis ATCC 49456 and cloned for construction of the recombinant protein. The recombinant MMP-like protein with a GST-tag was purified and the enzymatic activity was assessed. The recombinant protein showed approximately half the level of MMP-like activity as compared to human recombinant MMP-8. The MMP-like protein of S. mitis may be involved in the pathogenesis of infection to the dentin with collagen fibers and systemic invasive diseases.
Keywords:
Streptococcus mitis; Matrix metalloprotease; Collagen; Zinc-binding motif
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Copyright © 2020 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0