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eISSN: 2581-9615 || CODEN: WJARAI || Impact Factor 8.2 ||  CrossRef DOI

Research and review articles are invited for publication in April 2026 (Volume 30, Issue 1) Submit manuscript

Identification of MMP-like protein from Streptococcus mitis

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  • Identification of MMP-like protein from Streptococcus mitis

Hiroaki Aso 1, *, Hiroshi Maeda 1, Takayuki Nambu 2, Toshinori Okinaga 2 and Muneyasu Shida 1

1 Department of Endodontics
2 Bacteriology Osaka Dental University 8-1 Kuzuhahanazonocho Hirakata, Osaka 573-1121, Japan.
 
Research Article
World Journal of Advanced Research and Reviews, 2020, 08(03), 154-161
Article DOI: 10.30574/wjarr.2020.8.3.0473
DOI url: https://doi.org/10.30574/wjarr.2020.8.3.0473
 
Received on 04 December 2020; revised on 11 December 2020; accepted on 13 December 2020
 
Streptococcus mitis is a normal commensal of the human oral cavity and oropharynx. This microorganism is an opportunistic pathogen in immune compromised hosts and a cause of invasive diseases such as infective endocarditis. We isolated a matrix metalloprotease (MMP)-like protein of S. mitis. The gene for the MMP-like protein was found on the genome sequence database of S. mitis . The gene encodes a protein consisting of 240 amino acid residues with a conserved zinc-binding motif (HEXXHXXGXXH) among the matrix metallopeptidase family. The gene was PCR-amplified from S. mitis ATCC 49456 and cloned for construction of the recombinant protein. The recombinant MMP-like protein with a GST-tag was purified and the enzymatic activity was assessed. The recombinant protein showed approximately half the level of MMP-like activity as compared to human recombinant MMP-8. The MMP-like protein of S. mitis may be involved in the pathogenesis of infection to the dentin with collagen fibers and systemic invasive diseases. 
 
Streptococcus mitis; Matrix metalloprotease; Collagen; Zinc-binding motif
 
https://wjarr.com/sites/default/files/fulltext_pdf/WJARR-2020-0473.pdf

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Hiroaki Aso, Hiroshi Maeda, Takayuki Nambu, Toshinori Okinaga and Muneyasu Shida. Identification of MMP-like protein from Streptococcus mitis. World Journal of Advanced Research and Reviews, 2020, 8(3), 154-161. Article DOI: https://doi.org/10.30574/wjarr.2020.8.3.0473

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