College of health and medical techniques, Middle technical university, Baghdad.
Research Article
World Journal of Advanced Research and Reviews, 2022, 15(01), 683-693
Article DOI: 10.30574/wjarr.2022.15.1.0758
Received on 23 June 2021; revised on 26 July 2022; accepted on 28 July 2022
Introduction: A total of (116) diarrhea-sufficient patient samples gathered from February 15, 2019 to April 15, 2019.
Methodology: Using colonial morphology, microscopic examination, biochemical testing, and the 20 R API Enter systems, the bacteria were able to be identified. Ultimate "PCR" identification of E. coli ("MDH" gene).
Result: Rendering to the results obtained according to the morphological, cultural and biochemical characters From the 116 clinical specimen only 42(59.4%) isolates were belonged to E. coli. PCR analysis of the MDH gene (392bp) showed that 26/42(61.9%) were positive.
Using the Polymerase chain reaction (PCR) assay the bla-TEM-1 gene11/42(26.19%) of E. coli , were carrying of blaTEM-1 genes was as well as 30/42(71.4%) of E. coli using universal bla-CTX-M primers. Also examined were the antimicrobial susceptibility patterns of the isolates, occurred between 0.00% and 100.00%.
Conclusions: The present study revealed that of the rate bacterial infection which causes diarrhea is more causative agent and the higher infection rate among 1month – 2 years age than other age. In this study found the molecular technique method is more efficient than other methods such as (morphology, biochemical and API20E). There was a significant prevalence of ESBL-producing bacterial isolates, and the medicines ciprofloxacin and imipenem were very effective against bacteria isolates. The high prevalence of sul-1 gene than other genes (bla CTX-M and bla TEM).
Diarrhea; API Enteric System 20 R; Patient; E. coli
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Wafaa Fadhil Hamad. Genotypic and phenotypic study of E. coli isolated from children suffering from severe diarrhea with some antibiotic resistant gene. World Journal of Advanced Research and Reviews, 2022, 15(1), 683-693. Article DOI: https://doi.org/10.30574/wjarr.2022.15.1.0758