Enumerate chlorogenic acid in different forms of coffee by simple high performance thin layer chromatographic method
1Department of Pharmacology, R.G. Kar Medical College, Kolkata, India.
2RKM Vivekananda Educational & Research Institute, Kolkata, India.
3RKM Vivekananda Educational & Research Institute, Kolkata, India.
4Multidisciplinary Research Unit, R.G. Kar Medical College, Kolkata, India.
5Department of Biochemistry, R.G. Kar Medical College, Kolkata, India.
Research Article
World Journal of Advanced Research and Reviews, 2021, 09(02), 120-126
Article DOI: 10.30574/wjarr.2021.9.2.0051
Publication history:
Received on 12 January 2021; revised on 14 February 2021; accepted on 16 February 2021
Abstract:
The health benefit of coffee consumption mainly depends on chlorogenic acid (CGA), one of its major active phenolic constituent. CGA have several biological properties like anticancer, antihypertensive, hepatoprotective, neuroprotective, renoprotective, antimicrobial etc. It is assumed that different forms of coffee contains different amount of CGA. In this regards, we developed and validated a simple and cost effective chromatographic technique to identify and quantify CGA in green coffee, roasted coffee and branded instant coffee available in Indian market.
Keywords:
Coffee; Chlorogenic acid; HPTLC; UV-VIS spectroscopy
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