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eISSN: 2581-9615 || CODEN: WJARAI || Impact Factor 8.2 ||  CrossRef DOI

Research and review articles are invited for publication in March 2026 (Volume 29, Issue 3) Submit manuscript

An efficient in vitro callogenesis from various explants of Passiflora vitifolia kunth: An important medicinal and ornamental plant

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  • An efficient in vitro callogenesis from various explants of Passiflora vitifolia kunth: An important medicinal and ornamental plant

Chandana K.P 1, Hemanth Kumar N.K 2, *, Prithviraj H.S 3, Basavaraj G.L.4, Nataraj, K 2 and Shobha Jagannath 1

1 Department of Studies in Botany, University of Mysore, Manasagangotri, Karnataka, Mysuru-570006, India.
2 Department of Botany, Maharanis Science College for Women’s (Autonomous), JLB Road, Mysuru-570005, India.
3 Department of Botany, Sri Mahadeshwara Government First Grade College, Kollegal, Karnataka, India.
4 Department of Botany, Government College for Women (Autonomous), Mandya, Karnataka, India.\
 
Research Article
World Journal of Advanced Research and Reviews, 2022, 16(03), 723-729
Article DOI: 10.30574/wjarr.2022.16.3.1415
DOI url: https://doi.org/10.30574/wjarr.2022.16.3.1415
 
Received on 12 November 2022; revised on 21 December 2022; accepted on 24 December 2022
 
An efficient In vitro protocol was established for callogenesis from various plant parts (Leaf, stem, flower bud and tendril) of Passiflora vitifolia Kunth. On Murashige and Skoog (MS) basal medium supplemented with various concentration of auxins (NAA, IAA, 2,4-D) and cytokinins (BAP and Kn ). Different concentrations of auxins (0.5 to 4 mg/L), IAA (0.5 to 2mg/L), NAA (0.5 to 2 mg/l) and cytokinin BAP (0.5 to 2 mg/L), Kn (0.5 to 2 mg/L) were assessed for callus induction. Growth regulators when supplemented individually on MS medium produced hard white callus in stem and tendril explant, soft friable callus in leaf and flower bud explants. The callogenic response was more in leaf, stem and flower explant on MS media supplemented with NAA (1.5mg/L) and the callogenic response was more in tendril explants on MS media supplemented with 2,4-D (1mg/L).
 
Callus; Passiflora; Flower bud; Auxins
 
https://wjarr.com/sites/default/files/fulltext_pdf/WJARR-2022-1415.pdf

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Chandana K.P, Hemanth Kumar N.K, Prithviraj H.S, Basavaraj G.L., Nataraj, K and Shobha Jagannath. An efficient in vitro callogenesis from various explants of Passiflora vitifolia kunth: An important medicinal and ornamental plant. World Journal of Advanced Research and Reviews, 2022, 16(3), 723-729. Article DOI: https://doi.org/10.30574/wjarr.2022.16.3.1415

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