1Nature and Life sciences Department, Faculty of Nature and Life Sciences and Sciences of the Earth and the Universe, 08 May 1945 University, Guelma, Algeria.
2 Department of Agronomy, Faculty of Sciences of Nature and Life, Chadli Bendjedid University, El Tarf, Algeria.
3 Department of Biology, Faculty of Sciences of Nature and Life, Chadli Bendjedid University, El Tarf, Algeria.
4 Department of Biology Sciences, Faculty of Natural and Life Sciences and Sciences of Earth and The Universe, Mohamed El-Bachir El-Ibrahimi University, Bordj Bou Arreridj, Algeria.
5 Laboratory of Cellular Toxicology, Department of Biology, Faculty of sciences, Badji Mokhtar University, Annaba, Algeria.
Research Article
World Journal of Advanced Research and Reviews, 2020, 07(03), 133-141
Article DOI: 10.30574/wjarr.2020.7.3.0300
Received on 11 August 2020; revised on 06 September 2020; accepted on 12 September 2020
Xenoestrogens are artificial products that can be of industrial, agricultural or pharmaceutical origin. We have undertaken work to examine the possible effects of a stressor; a systemic fungicide considered to be an estrogen mimetic, Mancozebe, on tadpoles of an amphibian species; the green frog Rana saharica. After treatment for 5 weeks with increasing concentrations of Mancozeb (0.25, 0.50, 0.75 and 1 mg/l). In order to evaluate the biochemical and metabolic aspect and to know the details of the oxidative stress, we performed some assays. A disturbance of the anti-oxidant detoxification systems (Glutathion-S-Transferase GST and Catalase CAT) which are involved in the defense mechanisms against stress caused by Mancozeb was highlighted. We also found an increase in Malondialdehyde (MDA) levels due to lipid peroxidation and neurotoxicity confirmed by inhibition of Acetylcholinesterase (AchE) activity. The induction of oxidative stress prompted us to explore, among other things, the histological side and tissue damage caused by Mancozeb in tadpoles. Our results revealed hepatocyte necrosis and cytoplasmic vacuolation.
Oxidative stress; Xenoestrogen; Fungicide; Mancozeb; Tadpoles; Histology.
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Sana Benosmane, Amel Alayat, Zine Eddine Boumedris, Ouissem Moumeni, Houria Berrebah and Mohammed Reda Djebar. Effects of sublethal exposure of fungal xenoestrogen on oxidative stress and hepatic histology of tadpoles Rana saharica. World Journal of Advanced Research and Reviews, 2020, 7(3), 133-141. Article DOI: https://doi.org/10.30574/wjarr.2020.7.3.0300