Effect of the dose and duration of fermentation of catfish waste by a consortium of lipolytic microbes on microbiological quality and nutrient content

The purpose of the study was to explore the influence of the dose and duration of fermentation of a consortium of microbes, Pseudomonas aeruginosa, Rhizopus microspores, and Candida lipolytica that resulted in the microbiological quality (total microbes and temperature) and nutrient content (fat and protein content) of the best fermented catfish waste. The research was conducted experimentally in the laboratory using the Complete Random Design of the Nesting Pattern. The treatment consisted of inoculum doses (D1:5%, D2:10%, D3:15%, D4:20%) and fermentation duration (W1:2 days, W2:4 days, W3:8 days) with 3 repeats. The data from the study were tested using multiple fingerprints and continued with the Duncan multiple distance test. The results showed that the inoculum dose exerted a significant effect (P<0.05) on total microbes, fat, and protein levels. The fermentation time exerts a noticeable influence (P<0.05) on total microbes, temperature, fat, and protein content. The use of a dose of 5% inoculum and a fermentation period of 2 days resulted in the highest number of microbes, which is 216 × 10^9 CFU/mL, the highest protein is 59.15%, and the highest temperature is 30.37 °C. The use of a dose of 10% inoculum and a fermentation period of 2 days resulted in the lowest fat content of 42.46%. 

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