Effect of combined seeds of Sesemum indicum and Moringa oleifera compounded feed on liver function markers of alloxan-induced diabetic albino rats
1 Department of Biochemistry, Federal University of Technology, Owerri, Nigeria.
2 Department of Animal Science, Federal University of Technology, Owerri, Nigeria.
Research Article
World Journal of Advanced Research and Reviews, 2023, 19(02), 583–596
Publication history:
Received on 28 May 2023; revised on 05 August 2023; accepted on 08 August 2023
Abstract:
This study investigated the effect of combined seeds of Sesemum indicum (SI) and Moringa oleifera (MO) on liver function markers in alloxan-induced diabetic albino rats. 54 albino rats were divided into 9 groups of 6 animals each. Group 1 served as the normal control and received standard feed diet, Group 2 (Diabetic control) received intraperitoneal injection of 140mg/kg of Alloxan, Group 3 (Positive control) received 5mg/kg of glibenclamide, Group 4; diabetic rats fed diet containing 15% of SI. Group 5; diabetic rat fed with 15% of MO diet, Group 6; diabetic rat fed with combination of 15% SI and 15% MO compounded diet. Group 7; diabetic rat fed with 30% of SI diet. Group 8; diabetic rat fed with 30% of MO diet. Group 9; diabetic rat fed with combination of 30% MO and 30% SI diet. The Feed was compounded and was given daily for 14 days. Serum alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), total protein, albumin, and bilirubin were estimated to assess hepatic function using spectrophotometric method. Results showed a significant decrease in ALT, ALP, AST, and bilirubin levels when fed with compounded feeds at (p<0.05). Also feeding of diet with combined seeds significantly (P<0.05) increased the total protein and albumin in induced rats. Overall, compounded feed with combined seeds of SI and MO exhibited a promising anti-diabetic activity and normalized the liver enzyme activities which appeared comparable to the control glibenclamide in alloxan-induced diabetes hence, may be recommended for treatment/management of diabetes mellitus.
Keywords:
Anti-diabetic; Diabetes mellitus; Feed formulation; Moringa oleifera; Sesemum indicum
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