Effect of Bacillus subtilis and Bacillus coagulans Liquid Probiotics on Duodenum Histomorphology in Broiler

Introduction: Probiotics can nourish the digestive tract and increase nutrient digestibility so that nutrient intake is met for livestock. Bacillus subtilis and Bacillus coagulans as a prophylaxis against bacterial diseases by balancing intestinal microbes, increasing digestibility, and can secrete protease, lipase, and amylase enzymes. The purpose of this study was to determine the effect of probiotics Bacillus subtilis and Bacillus coagulans on duodenal histomorphology. 

Objective: Twenty-four broilers were randomly divided into 4 groups. The control group was not given probiotics, groups P1, P2, and P3 were given probiotics of 2 ml, 4 ml, and 6 ml. Probiotics were given for 14 days. One way ANOVA showed a significant difference in each groups (P<0.05) and continued with the Duncan test.

Results: The average length of the duodenal villi in each treatment showed that the control group was significantly different from the P1, P2, and P3 groups. The P3 group had the highest average length of duodenal villi. 

Conclusion: The conclusion from the research that has been done is that the use of Bacillus subtilis and Bacillus coagulans as probiotics has an effect on the broiler duodenal villi which is characterized by an increase in the average length of the duodenal villi according to the increase in the probiotic dose.