Development and characterization of Tinospora cordifolia extract-loaded SLNs for the treatment of autoimmune hepatitis
1 Department of Pharmacy, BBDIT, Duhai, Ghaziabad Uttar Pradesh, India.
2 Department of Pharmacy, Krishna Institute Bijnor, Near By -6 milestone Golbhag Chauraha Noorpur Road Bijnor (U.P) 246701, India.
3 Department of Pharmacy, Bansthali Vidyapith, Tonk Road Niwaru – 304022, India
4 Department of Pharmacy, Maharishi Markendeshwar College of Pharmacy, Mullana, Ambala, Haryana, India.
5 IIMT College of Medical Sciences, IIMT University, Ganga Nagar, Meerut, Uttar Pradesh, India – 250001, India.
6 Department of Pharmacy, Shivajirao S Jondhle College of Pharmacy, Asangaon, Thane Maharashtra. 421601, India.
7 Department of Pharmacy, Navsahyadri Institute of Pharmacy, Naigaon, Nasarapur, Pune, Maharashtra 412213, India.
8 College of Pharmaceutical Sciences, PIMS (DU), Loni (Bk)Tal Rahata, Dist. Ahmednagar, Maharashtra, India.
Research Article
World Journal of Advanced Research and Reviews, 2023, 20(03), 1102–1114
Article DOI: 10.30574/wjarr.2023.20.3.2565
Publication history:
Received on 30 October 2023; revised on 15 December 2023; accepted on 17 December 2023
Abstract:
Solid Lipid Nanoparticles (SLNs) have emerged as promising drug delivery systems with the potential to enhance the therapeutic efficacy of bioactive compounds. In this study, T. Cordifolia extract-loaded SLNs were developed and characterized for their application in the treatment of autoimmune hepatitis. T. Cordifolia, known for its immunomodulatory and anti-inflammatory properties, was chosen as the active ingredient. The formulations were systematically evaluated for drug-excipient compatibility, particle size, zeta potential, drug loading efficiency, drug release kinetics, encapsulation efficiency, in-vitro release, and stability. The results indicated "No Change" in drug-excipient compatibility, ensuring the formulation's stability. The SLNs exhibited nanoscale particle sizes (159.30 nm to 172.12 nm) with narrow size distributions, facilitating consistent drug delivery. Negative zeta potentials (-28.28 mV to -35.44 mV) indicated colloidal stability. High drug loading efficiencies (up to 32.23%) and controlled drug release kinetics were observed, suggesting the potential for sustained and targeted drug delivery. Encapsulation efficiencies of up to 83.41% highlighted efficient drug loading within the SLNs. In-vitro release studies revealed that SLN2 and SLN4 exhibited superior drug release profiles compared to other formulations. These findings indicate the potential of these formulations for controlled drug delivery. In-vivo efficacy studies in murine models of autoimmune hepatitis are recommended to assess the therapeutic benefits of T. Cordifolia extract-loaded SLNs. Additionally, stability studies demonstrated the maintenance of critical parameters, such as particle size, zeta potential, and drug loading efficiency, under different storage conditions.
Keywords:
Tinospora cordifolia; Solid Lipid Nanoparticles; Autoimmune hepatitis; Drug delivery; Immunomodulatory; Anti-inflammatory; Encapsulation efficiency; Drug release kinetics; Stability studies.
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