Iakob Gogebashvili Telavi State University, 1, Kartuli Universiteti str., Telavi, 2200, Georgia.
Research Article
World Journal of Advanced Research and Reviews, 2024, 23(02), 939-943
Received on 30 June 2024, revised on 07 August 2024, and accepted on 10 August 2024
The standard system has been elaborated for the control of lytic activity of the commercial series - active coli-bacteriophage and respective stable E. coli strains' collection. Special investigations were carried out with an aim to obtain polyvalent bacteriophage, which should comply with the requirements for the standard preparations. While passing of the active phage on the highly reproducible standard strain the coli-bacteriophage phage-lysate was obtained. With an aim to preserve its basic properties the phage-lysate has been lyophilized. The 20% Difco-peptone was used as the stabilizer. The lyophilized bacteriophage completely satisfies the parameter standards for biological preparations: it has high lytic activity, wide range of action, and thermal resistance. These properties are reliably preserved for the period of three years. It was shown experimentally that the lyophilized cultures could be used for evaluation of the lytic activity in bacteriophages. The identical phage-sensitivity was revealed in both lyophilized- and agar-cultures.
E. coli strains; Coli-Bacteriophage; Stabilizers; Lyophilization; Branch Standard Specimen
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Davitashvili Magda, Zuroshvili Lamara and Natsvlishvili Nana. Determination of lytic activity of coli-bacteriophage. World Journal of Advanced Research and Reviews, 2024, 23(2), 939-943. Article DOI: https://doi.org/10.30574/wjarr.2024.23.2.2415