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eISSN: 2582-8185 || CODEN: WJARAI || Impact Factor 8.2 ||  CrossRef DOI

Research and review articles are invited for publication in March 2026 (Volume 29, Issue 3) Submit manuscript

Design of primers for evaluation of some gram-negative bacteria isolated from intensive care unit patients in Wasit province, Iraq

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  • Design of primers for evaluation of some gram-negative bacteria isolated from intensive care unit patients in Wasit province, Iraq

Rana Essa Muslem and Rana H. Raheema *

Department of Medical Microbiology, Faculty of Medicine, University of Wasit, Iraq.
 
Research Article
World Journal of Advanced Research and Reviews, 2022, 16(02), 825-832
Article DOI: 10.30574/wjarr.2022.16.2.1133
DOI url: https://doi.org/10.30574/wjarr.2022.16.2.1133
 
Received on 25 September 2022; revised on 11 November 2022; accepted on 14 November 2022
 
Intensive care unit (ICU) patients frequently have consequences from healthcare-associated infections (HAIs), which include bacteremia, pneumonia, urinary tract, skin, or soft tissue infections. . In this study, a total of 100 clinical specimens (urine, sputum and pus) were collected from patients admitted in the ICU. Results showed eighty two were positive growth culture ‎and admitted to the intensive care unit distributed 1 (1.2%) Klebsiella oxytoca, 5(6.25%)‎Proteus mirabilis, 2 (2.4%)‎ Acinetobacter baumannii , 1 (1.2%)‎ Serratia marcescens and 1 (1.2%)‎ Burkholderia cepacia group bacteria . The results of this study showed that the diagnostic test for 16S rRNA ‎bacteria (Proteus mirabilis), which numbered 5 (100%), bacteria ‎‎(Acinetobacter baumannii), which numbered 2 (100%), bacteria ‎‎(Burkholderia cepacia ), which numbered 1 (100%), bacteria ‎‎(Serratia marcescens ), which numbered 1 (100%), and Bacteria ‎‎(klebsiella oxytoca ), which numbered 1 (100%) after completing the ‎phenotypic and biochemical diagnosis diagnostic test (16S rRNA).
 
16S rRNA; Acinetobacter baumannii; Proteus mirabilis; Serratia marcescens; Klebsiella oxytoca; Burkholderia cepacia complex; Intensive care unit
 
https://wjarr.com/sites/default/files/fulltext_pdf/WJARR-2022-1133.pdf

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Rana Essa Muslem and H. Raheema. Design of primers for evaluation of some gram-negative bacteria isolated from intensive care unit patients in Wasit province, Iraq. World Journal of Advanced Research and Reviews, 2022, 16(2), 725-832. Article DOI: https://doi.org/10.30574/wjarr.2022.16.2.1133

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