Design of primers for evaluation of some gram-negative bacteria isolated from intensive care unit patients in Wasit province, Iraq
Department of Medical Microbiology, Faculty of Medicine, University of Wasit, Iraq.
Research Article
World Journal of Advanced Research and Reviews, 2022, 16(02), 825-832
Article DOI: 10.30574/wjarr.2022.16.2.1133
Publication history:
Received on 25 September 2022; revised on 11 November 2022; accepted on 14 November 2022
Abstract:
Intensive care unit (ICU) patients frequently have consequences from healthcare-associated infections (HAIs), which include bacteremia, pneumonia, urinary tract, skin, or soft tissue infections. . In this study, a total of 100 clinical specimens (urine, sputum and pus) were collected from patients admitted in the ICU. Results showed eighty two were positive growth culture and admitted to the intensive care unit distributed 1 (1.2%) Klebsiella oxytoca, 5(6.25%)Proteus mirabilis, 2 (2.4%) Acinetobacter baumannii , 1 (1.2%) Serratia marcescens and 1 (1.2%) Burkholderia cepacia group bacteria . The results of this study showed that the diagnostic test for 16S rRNA bacteria (Proteus mirabilis), which numbered 5 (100%), bacteria (Acinetobacter baumannii), which numbered 2 (100%), bacteria (Burkholderia cepacia ), which numbered 1 (100%), bacteria (Serratia marcescens ), which numbered 1 (100%), and Bacteria (klebsiella oxytoca ), which numbered 1 (100%) after completing the phenotypic and biochemical diagnosis diagnostic test (16S rRNA).
Keywords:
16S rRNA; Acinetobacter baumannii; Proteus mirabilis; Serratia marcescens; Klebsiella oxytoca; Burkholderia cepacia complex; Intensive care unit
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